Anti-Septin-6 Rabbit Polyclonal Antibody for WB and ELISA - Q14141

Core Product Specifications and Parameters

Biological Background: Septin-6 Function and Localization

• Septin-6 is a member of the septin family of filament-forming cytoskeletal GTPases, essential for normal organization of the actin cytoskeleton, cytokinesis, and various cell cycle-related processes.
• It also may facilitate hepatitis C virus (HCV) RNA replication, highlighting a role in host-virus interactions.
• Subcellularly, Septin-6 is located in the cytoplasm, cytoskeleton (spindle, centromere/kinetochore, cleavage furrow, midbody), and cell projections including cilia and flagella.
• Widely expressed across tissues, Septin-6 is critical for spermatogenesis; it forms a filamentous structure with SEPTIN12, SEPTIN2, and likely SEPTIN4 at the sperm annulus, essential for sperm tail integrity and motility during postmeiotic differentiation. Related references: PMID:25588830
• The protein undergoes post-translational modifications such as acetylation and phosphorylation, with a predicted molecular weight of approximately 50 kDa (UniProt: 49.7 kDa).
• The SEPTIN6 gene (aliases: KIAA0128, SEP2, SEPT6) produces alternative spliced isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to the C-terminal region (aa 350-434) of human Septin-6; it may detect endogenous Septin-6 and potentially cross-react with isoforms containing this region.
• For Western blot (WB) analysis, consider testing the antibody in relevant cell or tissue lysates from human, mouse, or rat, as cross-reactivity has been reported for these species.
• In ELISA applications, optimize coating and detection conditions, and include appropriate controls to confirm specificity.
• Due to the presence of multiple isoforms, confirm band patterns by using positive controls or knockdown/knockout lysates if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, connecting diagnostic manufacturers and research labs with validated antibodies like this anti-Septin-6 polyclonal antibody. Whether you need bulk quantities of ancillary reagents, optimized antibody pairs, or custom sourcing for rare targets in the cytoskeleton and cell division research fields, CamelBio streamlines your procurement from concept to clinic. Our expertise supports seamless integration of high-quality raw materials into your diagnostic and research workflows.