Anti-Cytokeratin 7 (KRT7) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P08729

Core Product Specifications and Parameters

Biological Background: Keratin 7 (Cytokeratin 7/KRT7) Function and Localization

• Keratin 7 (KRT7), also known as cytokeratin-7 (CK-7), sarcolectin, or type-II keratin Kb7, is a member of the type II keratin family, which forms intermediate filament cytoskeletal networks essential for epithelial cell structural integrity.
• KRT7 pairs with type I keratins (e.g., KRT19) to assemble heterodimers that polymerize into 10 nm intermediate filaments, maintaining cytoplasmic architecture and mechanical resilience in simple and glandular epithelia.
• Unconventionally, KRT7 blocks interferon-dependent interphase and stimulates DNA synthesis, suggesting a regulatory role in cell cycle progression beyond cytoskeletal functions.
• KRT7 is involved in the translational regulation of human papillomavirus type 16 (HPV16) E7 mRNA, implicating it in host-virus interactions and oncogenic pathways.
• The protein is predominantly localized in the cytoplasm (UniProt: P08729), consistent with its structural and non-canonical cytoplasmic activities.
• Key post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination, which may modulate its stability, filament assembly, and protein interactions; it is also a glycoprotein and contains isopeptide bonds.
• Tissue expression is restricted: KRT7 is observed in cultured epidermal, bronchial, and mesothelial cells, and in glandular cells of the gastroesophageal junction, but is absent in colon, ectocervix, liver, and esophagus, making it a useful lineage marker.
• Additional keywords: intermediate filament, coiled coil, host-virus interaction, proteomics identification, reference proteome, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 230–469 of human KRT7 (NP_005547.3), targeting an internal region of the protein; this design may facilitate detection of full-length KRT7 in denaturing western blot conditions.
• For western blot (WB), a band around 51 kDa is expected; include appropriate positive controls (e.g., lysates from epithelial cell lines known to express KRT7) and negative controls (e.g., colon or liver tissues, which lack KRT7).
• In immunofluorescence (IF-P), optimize fixation and permeabilization protocols to preserve cytoplasmic intermediate filament structure; co-staining with a nuclear dye (e.g., DAPI) can confirm subcellular localization.
• The antibody has been shown to cross-react with mouse and rat KRT7 orthologs; however, sequence homology should be verified, and validation in the species of interest is recommended.
• For ELISA, pair with a suitable detection antibody or use as a capture reagent; titration experiments are advised to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-KRT7 pAb, to support epithelial biology research and diagnostic development. From antibody pairs and bulk ancillary reagents to rare target sourcing, our one-stop platform streamlines procurement for diagnostic manufacturers, laboratories, and research institutes. Explore our catalog or contact our technical team to discuss customized solutions for your KRT7-based assays.