Anti-Ly-6G Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P35461

Core Product Specifications and Parameters

Biological Background: Ly-6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also referred to as Ly-6G.1 and Gr-1, is encoded by the Ly6g gene in mouse (GeneID: 546644).
• Ly-6G is a cell membrane protein attached via a glycosylphosphatidylinositol (GPI) anchor, as indicated by UniProt annotations.
• Expression of Ly-6G is predominantly detected in bone marrow.
• Key structural features include a signal peptide, an extracellular domain containing a disulfide bond, and N-linked glycosylation sites, as annotated in UniProt (Disulfide bond, Glycoprotein, Signal).
• The calculated molecular weight of the Ly-6G proprotein is approximately 14.3 kDa (UniProt), and the antibody detects a band at around 14 kDa, consistent with the mature membrane-bound form.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 27–105) represents the mature extracellular domain of Ly-6G, making this antibody suitable for detecting surface-expressed protein in techniques like immunofluorescence on live cells (IF-P).
• For Western blotting, expect a specific band near 14 kDa. Use bone marrow cell lysates as a positive control due to Ly-6G’s expression profile.
• As a polyclonal antibody raised in rabbit, it will require appropriate anti-rabbit secondary antibodies for detection. Validate cross-reactivity carefully in your specific model system.
• For ELISA applications, consider pairing this antibody with a capture antibody targeting a different epitope to maximize sensitivity in sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to empowering diagnostic developers with end-to-end access to IVD raw materials, technical guidance, and consulting—from concept to clinic. This anti-Ly-6G antibody is part of our broad portfolio supporting leukocyte and myeloid biology research. We source validated antibody pairs, custom monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay pipeline.