Rabbit anti-Mouse FcγRI/CD64 Monoclonal Antibody for Flow Cytometry - P26151

Core Product Specifications and Parameters

Biological Background: Mouse FcγRI/CD64 Function and Localization

• High affinity receptor for the Fc region of immunoglobulins gamma, playing a critical role in both innate and adaptive immune responses.
• Member of the immunoglobulin superfamily, functioning as an IgG-binding protein essential for antibody-mediated effector functions.
• Expressed specifically on macrophages, where it mediates antibody-dependent cellular phagocytosis and cytokine release.
• Localized at the cell membrane, featuring a single-pass transmembrane domain and a signal peptide for surface expression.
• Post-translational modifications include glycosylation and disulfide bond formation; also contains phosphorylation sites.
• Classified under keywords: Disulfide bond, Glycoprotein, IgG-binding protein, Immunity, Immunoglobulin domain, Innate immunity, Membrane, Phosphoprotein, Receptor, Repeat, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• Due to its monoclonal nature, titrate the antibody to determine the optimal concentration for your specific flow cytometry assay.
• The immunogen spans amino acids 25–297, covering the extracellular domain, which suggests recognition of the native surface receptor.
• is a bright fluorophore suitable for multicolor panels; validate its compatibility with your instrument and compensation settings.
• Cross-reactivity is confirmed for mouse; ensure that other species are tested if used in heterologous systems.
• For intracellular detection, verify that the epitope is preserved under fixation and permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories streamlined access to a comprehensive portfolio of immune-related raw materials, including this anti-mouse FcγRI/CD64 antibody. Our offerings encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting your IVD development from concept to clinic.