Rabbit Anti-Mouse CD68 Monoclonal Antibody for Flow Cytometry - P31996

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68, also known as macrosialin, Lamp4, gp110, or Scard1, is a heavily glycosylated transmembrane protein belonging to the LAMP family. Alternative splicing produces multiple isoforms.
• It functions in phagocytosis by macrophages, participating in intracellular lysosomal metabolism and extracellular interactions with pathogens and other cells.
• CD68 binds tissue- and organ-specific lectins or selectins, facilitating the homing of macrophage subsets to particular sites.
• The protein rapidly recycles between endosomes, lysosomes, and the plasma membrane, which may enable macrophage crawling on selectin-coated substrates.
• Subcellular localization includes endosome membrane, lysosome membrane, and cell membrane.
• Expressed predominantly in tissue macrophages and to a lesser extent in dendritic cells, serving as a classical macrophage marker.
• Key structural features include a signal peptide, a transmembrane helix, and multiple disulfide bonds that contribute to its stability.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, optimize fixation and permeabilization protocols for your specific sample type to ensure proper access to the intracellular epitope.
• The immunogen spans amino acids 21–291 of mouse CD68 (luminal domain); consider that the antibody may detect both glycosylated and unglycosylated forms of the protein.
• Validate the antibody in a relevant mouse tissue or macrophage cell line expressing CD68, using appropriate negative controls (e.g., non-macrophage cells or knockout samples).
• When performing multi-color flow cytometry, verify spectral compatibility of with other fluorophores in your panel.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to high-quality IVD raw materials, technical services, and consulting from concept to clinic. This anti-CD68 antibody targets a key macrophage marker widely used in immunology and inflammation research, and CamelBio can support your projects with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. Let us help streamline your assay development with reliable, scalable material supply.