Mouse anti-Rabbit IgG (Fc) Monoclonal Antibody for FC - P01870

Core Product Specifications and Parameters

Biological Background: Rabbit IgG (Fc) Function and Localization

• Rabbit Ig gamma chain C region (Fc fragment) is the constant portion of immunoglobulin heavy chains, mediating antibody effector functions such as complement activation and binding to Fc receptors.
• It contains multiple immunoglobulin-like domains, as indicated by the UniProt keyword 'Immunoglobulin domain', contributing to its stable beta-sandwich fold.
• The protein's molecular weight is approximately 35404 Da (35.4 kDa), consistent with the product's listed 35 kDa, based on direct protein sequencing data.
• The 'Repeat' keyword suggests the presence of internal structural repeats typical of immunoglobulin constant regions.
• As a reference proteome component, its sequence is fully validated by direct protein sequencing and 3D-structure determination, ensuring reliable antigen design.
• The Fc region is non-variable, making it an excellent target for secondary antibodies in immunological assays, with high specificity across rabbit IgG subclasses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the Fc fragment (aa 96-323), ensuring specificity for the constant region without cross-reactivity to light chains or Fab fragments; consider this when designing multiplex assays.
• This conjugate is bright and photostable, suited for flow cytometry; titrate carefully to achieve optimal signal-to-noise in your specific cell or bead system.
• Validate antibody performance in the appropriate species context (rabbit) as cross-reactivity with other species is not specified; inclusion of proper isotype controls is recommended.
• For intracellular staining, ensure adequate permeabilization without compromising the fluorophore; is compatible with common fixation/permeabilization buffers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-rabbit IgG (Fc) antibody enables robust detection of rabbit immunoglobulins, supporting immunoassay development and quality control workflows. From concept to clinic, we bridge your research needs with reliable, scalable sourcing solutions.