Mouse Anti-Rabbit IgM (Fc) Monoclonal Antibody for Flow Cytometry - P03988

Core Product Specifications and Parameters

Biological Background: Rabbit IgM Fc Region Function and Localization

• The rabbit immunoglobulin mu heavy chain constant region (Fc) is encoded by the IGHM gene, with the secreted form (P03988) lacking a transmembrane domain.
• It serves as the Fc portion of IgM antibodies, mediating effector functions such as complement activation and interaction with Fc receptors.
• The IgM antibody is the first immunoglobulin class produced during a primary immune response, forming pentamers linked by a J chain.
• Subcellular localization: Secreted into the bloodstream and mucosal sites, contributing to humoral immunity.
• Domains: Composed of four immunoglobulin-like constant domains (CH1-CH4) that facilitate multimerization and effector binding.
• Post-translational modifications: Multiple disulfide bonds stabilize the Ig fold, and N-linked glycosylation occurs at conserved residues, influencing stability and function.
• Alternative splicing produces both a membrane-bound form (part of the B cell receptor) and the secreted form (P03988).

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 107-458 of the rabbit IgM Fc region, ensuring specificity for the constant domain without cross-reacting with variable regions or other immunoglobulin classes.
• This antibody is directly conjugated to , a dye with excitation/emission maxima at ~650/670 nm, making it well-suited for flow cytometry using 633–640 nm laser lines.
• For flow cytometry, use appropriate isotype controls (mouse IgG matched to the conjugate) to assess background.
• Validate antibody performance in your specific sample type (e.g., rabbit lymphocytes, serum) and include relevant positive/negative controls.
• Consider the secreted nature of the target: for cell-surface staining ensure sufficient membrane-bound IgM is present or use fixation/permeabilization if intracellular detection is intended.
• Avoid prolonged exposure to light to maintain fluorophore brightness; store and handle according to the supplier’s recommendations (typically 4°C, protected from light).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner for diagnostic manufacturers, laboratories, and research institutes. This anti-rabbit IgM Fc monoclonal antibody is a reliable component for flow cytometry-based assays and immunoassay development targeting rabbit antibody detection. Beyond this product, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to support your entire diagnostic workflow from concept to clinic.