Rabbit anti-Mouse IgM Monoclonal Antibody for FC - P01872

Core Product Specifications and Parameters

Biological Background: IgM Function and Localization

• Immunoglobulin heavy constant mu (Ighm) encodes the constant region of IgM antibodies, which exist as membrane-bound B cell receptors (BCR) or secreted immunoglobulins. Secreted IgM mediates humoral immunity by binding antigens and triggering effector functions.
• Secreted IgM (sIgM) forms high-order multimers, primarily pentamers, allowing multivalent antigen recognition. Both natural and immune sIgM contribute to effective immune responses, with natural sIgM recognizing conserved structures and immune sIgM being antigen-specific. Related references: PMID:10899913, PMID:28135254, PMID:34788614
• IgM mediates its effector functions through Fc receptor (FCMR) binding and complement activation. It recruits C1q to initiate the classical complement pathway, promoting phagocytosis of pathogens and clearance of apoptotic cells. Related references: PMID:10899913, PMID:11062505, PMID:28135254
• Membrane-bound IgM (mIgM) constitutes the BCR complex and provides tonic signaling essential for B cell survival. It also regulates pre-BCR signaling and Ig gene rearrangement via allelic exclusion.
• In mucosal immunity, IgM is transported across epithelial cells by the polymeric immunoglobulin receptor (PIGR). IgM-antigen complexes undergo FCMR-mediated retrotranscytosis across M cells to underlying lymphoid tissues. Related references: PMID:34788614
• IgM is localized at the cell membrane and secreted into extracellular spaces. It is expressed in bone marrow and in B-1/B-2 cell populations.
• Post-translational modifications include glycosylation and disulfide bond formation (UniProt keywords: Glycoprotein, Disulfide bond). Alternative splicing generates membrane-bound and secreted isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 105–454 of mouse IgM (P01872), covering a large portion of the heavy chain constant region. This region is conserved across IgM but distinct from other immunoglobulin isotypes, suggesting the antibody is specific for IgM and unlikely to cross-react with IgG, IgA, etc.
• Validate the antibody's performance in your specific flow cytometry protocol by titrating to determine the optimal concentration for your cell type and fluorophore detector settings. Include appropriate isotype controls and viability dyes.
• Typical sample sources for mouse IgM detection are splenocytes, bone marrow, or peripheral blood. Proper cell handling and Fc receptor blocking are recommended to reduce nonspecific staining.
• When designing multicolor panels, account for the fluorophore's spectral properties and confirm compatibility with other conjugates to avoid compensation issues.
• Although the immunogen suggests native conformation recognition, users should verify reactivity in alternative applications (e.g., Western blot, IHC) through independent validation.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to validated antibody reagents, technical expertise, and supply chain solutions. Our portfolio includes this anti-IgM monoclonal antibody for B-cell immunity studies, and we can assist with sourcing additional antibodies, ancillary reagents, and custom development services to accelerate your immunological assay workflows. Contact our team to discuss your specific project needs.