Anti-Mouse CD32 Rabbit Monoclonal Antibody for IHC-P, FC, ELISA - P08101

Core Product Specifications and Parameters

Biological Background: CD32 Function and Localization

• CD32, also known as Fc gamma receptor IIB (FcγRIIB), is a low-affinity receptor for the Fc region of immunoglobulin gamma (IgG), playing a central role in immune complex binding and internalization.
• Encoded by the Fcgr2 gene (also known as Fcgr2b or Ly-17) in mouse, this protein contains immunoglobulin-like domains and exists in multiple isoforms (IIB1, IIB1', IIB2, IIB3) generated by alternative splicing.
• CD32 mediates a variety of effector and regulatory functions, including phagocytosis of immune complexes, modulation of antibody production by B-cells, and down-regulation of B-cell, T-cell, and mast cell activation when co-aggregated with BCR, TCR, or other Fc receptors.
• The isoforms exhibit functional differences: isoform IIB1 and IIB1' can form caps but do not mediate endocytosis or phagocytosis, whereas isoform IIB2 facilitates endocytosis of soluble immune complexes via clathrin-coated pits.
• The protein is widely expressed by cells of hematopoietic origin; isoform IIB1 is preferentially expressed in lymphoid lineage cells, isoform IIB2 in myeloid lineage cells, and isoform IIB3 is secreted by macrophages and detected in serum.
• CD32 localizes to the cell membrane, cytoplasm, and cytoskeleton; certain isoforms are secreted, consistent with its transmembrane and secreted forms.
• Post-translational modifications such as glycosylation, phosphorylation, and disulfide bond formation are critical for its structural integrity and function.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 30-207), which is shared across all isoforms; consider confirming isoform specificity in your experimental context if differential detection is required.
• For flow cytometry, use appropriate isotype controls and perform titration to determine the optimal antibody concentration for your cell population.
• In IHC-P, antigen retrieval may be necessary; validate staining patterns against the known tissue distribution of CD32 in hematopoietic tissues.
• For ELISA, the antibody can be used to detect recombinant or native CD32; optimize coating conditions and detection format for your specific assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This anti-mouse CD32 antibody supports research in immune modulation, autoimmune disease, and therapeutic antibody mechanisms. Let CamelBio streamline your supply chain from concept to clinic with tailored technical and procurement solutions.