Anti-CD64 Rabbit Monoclonal Antibody for WB, ELISA - P12314

Core Product Specifications and Parameters

Biological Background: CD64 Function and Localization

• CD64, also known as FcγRI, is the high-affinity receptor for the Fc region of immunoglobulin gamma (IgG). It is encoded by the FCGR1A gene in humans and belongs to the immunoglobulin superfamily.
• This receptor plays a critical role in linking humoral and cellular immunity by binding IgG with high affinity, enabling monocytes and macrophages to recognize and eliminate IgG-opsonized targets.
• CD64 mediates potent effector functions, including antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells, thereby contributing to antiviral immune responses.
• The protein is composed of three extracellular immunoglobulin-like domains, a single transmembrane helix, and a short cytoplasmic tail. The immunogen used corresponds to the extracellular region (amino acids 16–288), covering the IgG-binding domains.
• CD64 is specifically expressed on the surface of monocytes and macrophages, consistent with its role in innate immune surveillance and antigen presentation.
• Post-translational modifications include N-linked glycosylation and phosphorylation. The observed molecular weight range (45–75 kDa) reflects glycosylation heterogeneity.
• Alternative splicing generates multiple isoforms, some of which may be secreted or function as soluble receptors.
• As a key innate immune receptor and a trigger for ADCC, CD64 is a validated biomarker for monocyte/macrophage activation and a therapeutic target in cancer immunotherapy.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the entire extracellular domain (aa 16–288), suggesting that this antibody likely recognizes the native conformation as well as denatured epitopes. For Western blot, the observed banding pattern may appear as a broad band or multiple bands between 45 and 75 kDa due to variable glycosylation; use appropriate protein loading and reducing conditions.
• For ELISA, consider using recombinant FcγRI protein or cell lysates from human monocytic cell lines (e.g., THP-1) as the antigen source. Validate assay specificity with isotype controls and blocking with excess human IgG.
• Since CD64 expression is restricted to monocytes/macrophages, ensure that tissue or cell samples are enriched for this population to obtain robust signals. In mixed leukocyte preparations, co-staining with CD14 can help confirm monocyte identity.
• The antibody is expected to cross-react with human CD64 but not with other Fcγ receptors (CD32, CD16). If broader reactivity is needed, test against recombinant proteins of different FcγR family members.
• Given its role in ADCC, this antibody may be useful in functional assays monitoring monocyte-mediated killing; however, its blocking or activating properties should be experimentally determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers, laboratories, and research institutes seeking reliable IVD raw materials. This anti-CD64 monoclonal antibody is suited for assays exploring monocyte/macrophage biology, IgG receptor function, and ADCC research. Beyond individual antibodies, CamelBio streamlines procurement of validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing, supporting your workflow from concept to clinic.