Rabbit anti-Mouse FcγRI/CD64 Monoclonal Antibody for Flow Cytometry - P26151

Core Product Specifications and Parameters

Biological Background: FcγRI/CD64 Function and Localization

• High affinity immunoglobulin gamma Fc receptor I, also referred to as FcγRI, CD64, or Fc-gamma RI, is a key immune receptor.
• It functions as a high-affinity receptor for the Fc region of immunoglobulin gamma (IgG), participating in both innate and adaptive immune responses.
• The receptor is localized to the cell membrane, anchoring it for extracellular ligand interaction.
• Expression is macrophage-specific, highlighting its role in professional phagocyte function.
• Characterized as a glycoprotein with disulfide bonds, a signal peptide, and phosphorylation sites; it contains immunoglobulin-like domains.

Experimental Guidance and Technical Tips

• This -conjugated antibody is designed for direct flow cytometry; consider titrating to determine the optimal concentration for your staining protocol.
• Validate the antibody in appropriate mouse macrophage cell lines (e.g., RAW 264.7) or primary macrophages, using relevant isotype controls.
• To minimize non-specific binding, use Fc blocking reagents prior to staining.
• The immunogen spans the extracellular domain (amino acids 25-297), suggesting the antibody targets the ligand-binding region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive, one-stop platform for IVD raw-material sourcing, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-mouse FcγRI/CD64 monoclonal antibody supports immunology and immune-profiling applications, and CamelBio can assist in sourcing rare target raw materials along with technical consulting to streamline your assay development from concept to clinic.