Rabbit anti-Mouse FcγRI/CD64 Monoclonal Antibody for Flow Cytometry - P26151

Core Product Specifications and Parameters

Biological Background: FcγRI/CD64 Function and Localization

• FcγRI, also designated CD64, is a high-affinity receptor for the Fc region of immunoglobulin gamma (IgG). It is a key component linking humoral and cellular immunity.
• It functions in both innate and adaptive immune responses, mediating phagocytosis, antigen presentation, and release of inflammatory cytokines upon immune complex cross-linking.
• Expression is predominantly macrophage-specific, underscoring its critical role in professional phagocytic clearance of opsonized targets.
• The mature protein is a type I transmembrane glycoprotein containing three extracellular Ig-like domains, a single transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include glycosylation, disulfide bond formation within Ig domains, and phosphorylation at intracellular residues, suggesting regulatory potential.
• The extracellular region (residues 25-297) used as immunogen covers the IgG-binding domains, enabling the antibody to recognize the native receptor on live cells.
• Keywords assigned by UniProt: Cell membrane, disulfide bond, glycoprotein, IgG-binding protein, immunity, innate immunity, phosphoprotein, receptor, transmembrane, transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the entire extracellular portion of mouse CD64, favoring antibody recognition of the native receptor on the cell surface without permeabilization.
• direct conjugation enables one-step flow cytometry staining; titrate the antibody to determine the optimal concentration for your specific cell system.
• Include appropriate controls: isotype-matched control and Fc receptor blocking reagents (e.g., anti-CD16/CD32) to minimize non-specific binding.
• Since CD64 is internalized upon ligand binding, account for this dynamics if studying immune complex uptake; staining at 4 °C in the presence of sodium azide can inhibit receptor turnover.
• Consider macrophage activation status when interpreting CD64 expression levels, as inflammatory signals can modulate receptor density.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials and technical support—from concept to clinic. This monoclonal antibody targeting CD64 serves flow cytometry workflows and immune profiling studies. Through our validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, CamelBio accelerates your IVD development pipeline.