Mouse anti-Rabbit IgG (Fc) mAb for Flow Cytometry - P01870

Core Product Specifications and Parameters

Biological Background: Rabbit IgG Fc Function and Localization

• The target protein is the Ig gamma chain C region (P01870), representing the constant part of the rabbit IgG heavy chain.
• It has a calculated molecular weight of approximately 35.4 kDa, consistent with the constant region of an immunoglobulin gamma chain.
• The protein contains immunoglobulin C-type domains, characteristic of antibody constant regions that mediate structural stability.
• It features repeating immunoglobulin-like domain structures, a hallmark of antibody constant regions that contribute to overall architecture.
• Its three-dimensional structure has been experimentally determined, providing insights into its folding and interaction interfaces (Keywords: 3D-structure).
• The amino acid sequence has been verified by direct protein sequencing, confirming the accuracy of the annotated sequence (Keywords: Direct protein sequencing).
• It belongs to the rabbit reference proteome, serving as a standard model for immunoglobulin gamma chain studies (Keywords: Reference proteome).

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fragment spanning amino acids 96–323 of the rabbit IgG Fc region, which encompasses key constant domains; consider this design for intended epitope recognition.
• It is directly conjugated to , a bright fluorophore compatible with standard flow cytometry lasers; validate optimal titers in your specific sample and instrument setup.
• As a monoclonal antibody, it offers consistent lot-to-lot performance and low cross-reactivity; confirm specificity using appropriate rabbit-derived samples and isotype controls.
• The conjugate simplifies detection without the need for secondary reagents, reducing non-specific binding; however, ensure proper blocking and washing steps for optimal signal-to-noise.
• Given its reactivity with rabbit IgG Fc, it can be used to detect rabbit primary antibodies in indirect assays; verify compatibility with your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for IVD manufacturers and research labs by providing one-stop access to validated raw materials, including tailored monoclonal antibodies like this anti-rabbit IgG conjugate. With expertise in molecular biology and immunodiagnostics, we offer customized sourcing solutions for rare targets, bulk ancillary reagents, and optimized antibody pairs to support your workflow from concept to clinic. Partner with CamelBio to accelerate your rabbit IgG detection projects with reliable supply and technical guidance.