Rabbit anti-Human/Monkey CD64 Monoclonal Antibody for Flow Cytometry - P12314

Core Product Specifications and Parameters

Biological Background: CD64 (FcγRI) Function and Localization

• CD64 (FcγRI) is a high-affinity IgG Fc receptor encoded by the FCGR1A gene (also known as FCG1, FCGR1, IGFR1). It belongs to the immunoglobulin superfamily and contains three Ig-like domains.
• Functions as a key mediator of both innate and adaptive immune responses, primarily by binding the Fc region of IgG and triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells on monocytes/macrophages.
• Specifically expressed on monocytes and macrophages (monocyte/macrophage specific).
• Localized to the cell membrane as a single-pass type I membrane protein.
• Post-translationally modified by glycosylation and phosphorylation; annotated as a glycoprotein and phosphoprotein in UniProt.
• Subject to alternative splicing, generating multiple transcript variants.
• The unmodified protein has a calculated molecular weight of ~42.6 kDa; the product's observed band at 43 kDa likely reflects glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 16-288), making this antibody suitable for detecting surface-expressed CD64 by flow cytometry.
• Ensure proper titration for flow cytometry assays. Since CD64 is monocyte/macrophage specific, include appropriate cell lineage markers to gate on these populations.
• The conjugate provides a bright and photostable signal, suitable for multiplex panels. Validate in the relevant sample system, such as human PBMCs or cynomolgus samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-CD64 antibody supports immunology and flow cytometry applications, and CamelBio can assist with sourcing complementary reagents for complete assay development.