Rabbit anti-Mouse FcγRI/CD64 mAb - P26151

Core Product Specifications and Parameters

Biological Background: FcγRI/CD64 Function and Localization

• High affinity immunoglobulin gamma Fc receptor I (FcγRI), also known as CD64, is encoded by the Fcgr1 gene in mouse.
• Functions as a high affinity receptor for the Fc region of immunoglobulins gamma, playing a key role in both innate and adaptive immune responses.
• Expressed specifically on macrophages, aligning with its role in phagocytosis and antigen presentation.
• Located on the cell membrane as a single-pass transmembrane receptor.
• Contains immunoglobulin-like domains and is a glycoprotein with potential phosphorylation sites, indicating regulation by post-translational modifications.
• Participates in IgG binding and immune complex clearance, linking humoral and cellular immunity.

Experimental Guidance and Technical Tips

• For flow cytometry, use an appropriate isotype control and optimize antibody dilution for your cell population.
• Since CD64 is highly expressed on macrophages, consider gentle dissociation or blocking steps to reduce non-specific binding in adherent cell preparations.
• The immunogen covers the extracellular domain (amino acids 25–297), so the antibody should recognize the native surface receptor; validate in your specific mouse model or cell line.
• When multiplexing, check compatibility with other mouse markers and fluorophores to avoid spectral overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials for immunology research, including validated monoclonal antibodies like this anti-FcγRI/CD64 clone. We also offer ancillary reagents and custom sourcing for rare targets to accelerate your diagnostic development from concept to clinic. For researchers studying Fc receptor biology or macrophage-driven immune responses, CamelBio is your one-stop sourcing partner.