Mouse anti-Rabbit IgG (Fc) Monoclonal Antibody for FC - P01870

Core Product Specifications and Parameters

Biological Background: Rabbit IgG Fc Function and Localization

• The Ig gamma chain C region (UniProt: P01870) constitutes the constant region of the rabbit immunoglobulin heavy chain gamma, forming the Fc fragment of IgG antibodies which lack antigen-binding sites.
• It belongs to the immunoglobulin superfamily and contains highly conserved immunoglobulin-like domains, as classified under the keyword "Immunoglobulin domain".
• The primary structure of this region has been confirmed through direct protein sequencing, ensuring accurate characterization.
• Its three-dimensional structure has been experimentally determined, contributing to the keyword "3D-structure" and facilitating studies on antibody architecture.
• The Fc region mediates critical effector functions, including interactions with Fc receptors on immune cells to trigger responses such as antibody-dependent cellular cytotoxicity (ADCC) and complement cascade activation.
• The molecular weight of the expressed Fc fragment is approximately 35404 Da (calculated from UniProt), aligning with the observed ~35 kDa in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen used comprises the Fc fragment (amino acids 96-323) of rabbit IgG, strategically chosen to elicit antibodies highly specific to the constant region, minimizing cross-reactivity with other immunoglobulin isotypes.
• is a bright and photostable red fluorescent dye with excitation/emission suitable for standard flow cytometry lasers; consider optimizing the staining concentration by titration in your specific cellular system to achieve optimal signal-to-noise ratios.
• This conjugated monoclonal antibody is designed for direct flow cytometry detection of rabbit IgG, serving as a secondary reagent; ensure appropriate controls (e.g., cells lacking rabbit IgG, isotype controls) are included to validate specificity.
• The antibody’s mouse origin and monoclonal nature ensure consistent lot-to-lot performance; however, always confirm compatibility with blocking reagents and protocol buffers in advance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers centralized access to premium IVD raw materials, including validated monoclonal antibodies and ancillary reagents, supporting diagnostic developers from concept to clinic. For researchers focusing on immunoglobulin detection or developing rabbit IgG-based immunoassays, we supply fully characterized antibody pairs and bulk conjugates like this -conjugated antibody to streamline your workflows. Our expertise extends to sourcing rare-target reagents, ensuring your projects proceed without delays.