Rabbit anti-Mouse FcγRI/CD64 Monoclonal Antibody for FC - P26151

Core Product Specifications and Parameters

Biological Background: FcγRI/CD64 Function and Localization

• Encoded by the Fcgr1 gene in mouse, FcγRI (CD64) is a high-affinity receptor for the Fc region of immunoglobulin gamma (IgG), playing a pivotal role in both innate and adaptive immune responses.
• As a member of the immunoglobulin superfamily, it contains immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail, characteristic of activating Fc receptors.
• The protein is heavily glycosylated and contains disulfide bonds that stabilize its extracellular ligand-binding domains.
• It is specifically expressed on macrophages, where it mediates antibody-dependent cellular phagocytosis (ADCP) and cytokine release upon cross-linking by IgG-opsonized targets.
• Localized to the cell membrane, FcγRI initiates intracellular signaling through associated immunoreceptor tyrosine-based activation motifs (ITAMs), often via the common FcRγ chain.
• Post-translational modifications include phosphorylation, which modulates receptor signaling and internalization.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 25-297), suggesting the antibody likely recognizes the native conformation of the receptor, making it suitable for flow cytometry (FC) analysis.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type, using mouse macrophages (e.g., peritoneal macrophages or RAW 264.7 cell line) as positive controls.
• Pre-blocking with Fc block (anti-CD16/CD32) may be necessary to reduce non-specific binding to other Fc receptors when staining mouse cells.
• Note that the fluorescent label is sensitive to light; handle and store the antibody protected from light and avoid repeated freeze-thaw cycles.
• Validate the staining specificity by using isotype control or pre-incubating with unlabeled anti-CD64 antibody.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted partner for diagnostic and research communities, CamelBio offers integrated procurement of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this FcγRI/CD64 reagent. We support your immunology and oncology programs with bulk ancillary reagents and rare target sourcing, streamlining your path from concept to clinic.