Rabbit anti-Mouse FcγRI/CD64 Monoclonal Antibody for FC - P26151

Core Product Specifications and Parameters

Biological Background: FcγRI/CD64 Function and Localization

• FcγRI/CD64 is a high-affinity receptor for the Fc region of immunoglobulin gamma (IgG), playing a critical role in linking humoral and cellular immunity.
• Encoded by the Fcgr1 gene in mice, the protein is also known as Fc-gamma RI or FcRI.
• It is expressed specifically on macrophages, where it mediates phagocytosis, antigen presentation, and cytokine release upon immune complex binding.
• Subcellularly, CD64 resides on the cell membrane as a transmembrane glycoprotein.
• The protein contains immunoglobulin-like domains and features disulfide bonds and phosphorylation sites, contributing to its structure and signaling capacity.
• Predicted molecular weight of 44.9 kDa aligns with the observed 44 kDa band, consistent with potential glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 25-297) of mouse CD64, which contains the IgG-binding domains, suggesting the antibody may recognize the native receptor conformation.
• This antibody is validated for flow cytometry (FC) with mouse samples; direct conjugation to enables single-step staining and minimizes cross-species background.
• For FC, consider titrating the antibody to determine optimal staining concentration for your cell type; macrophages are the primary target, but other myeloid cells may also express CD64 upon activation.
• As CD64 is a transmembrane receptor, cell surface staining does not require permeabilization; use appropriate Fc blocking reagents to reduce non-specific binding to Fc receptors.
• Since the antibody is rabbit monoclonal, it offers batch-to-batch consistency for longitudinal studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, providing validated antibodies, bulk reagents, and rare target solutions. This Rabbit anti-Mouse FcγRI/CD64 monoclonal antibody is part of our portfolio of reliable diagnostic-grade materials, ensuring seamless transition from research to clinical assay development. For immunology and inflammation research, we offer a range of CD64-specific reagents, including conjugated antibodies for flow cytometry and ELISA antibody pairs, all supported by expert technical consultation.