Rabbit anti-Mouse CD68 Monoclonal Antibody for IF/ICC, FC - P31996

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68 (Macrosialin) is a heavily glycosylated transmembrane protein belonging to the lysosomal-associated membrane protein (LAMP) family.
• It plays a role in phagocytic activities, including intracellular lysosomal metabolism and extracellular cell-cell or cell-pathogen interactions.
• CD68 may bind tissue- and organ-specific lectins or selectins, enabling homing of macrophage subsets to particular sites.
• The protein rapidly recycles between endosomes, lysosomes, and the plasma membrane, a process that may facilitate macrophage crawling over selectin-bearing substrates.
• Subcellular localization includes endosome membrane, lysosome membrane, and cell membrane.
• Expressed predominantly in tissue macrophages and, to a lesser extent, dendritic cells.
• Features include a signal peptide, disulfide bonds, and alternative splicing that generates multiple isoforms.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF/ICC), consider permeabilization to access the predominantly endosomal and lysosomal pools of CD68; surface staining may also be detectable due to recycling.
• In flow cytometry (FC), a permeabilization step is often required to detect intracellular CD68; titrate the antibody to optimize signal-to-noise ratio.
• The immunogen spans the extracellular domain (aa 21-291), suggesting reactivity with both surface and intracellular forms; validate detection in your specific sample system.
• CD68 is a macrophage lineage marker; use appropriate isotype and cell-type controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop sourcing platform for IVD raw materials. Our catalog includes validated monoclonal antibodies like this anti-CD68 reagent, along with optimized antibody pairs, ancillary reagents, and sourcing services for rare targets. Whether you need macrophage markers for immunophenotyping or bulk supplies for kit development, CamelBio accelerates your project from concept to clinic with reliable supply chains and technical consultation.