Mouse anti-Rabbit IgG (Fc) Monoclonal Antibody for Flow Cytometry - P01870

Core Product Specifications and Parameters

Biological Background: Rabbit IgG (Fc) Structure and Keywords

• The target is the Ig gamma chain C region (UniProt P01870), the constant region fragment of rabbit IgG heavy chain comprising amino acids 96–323.
• It features immunoglobulin domain(s) and internal repeats, characteristic of the immunoglobulin superfamily.
• The predicted molecular weight of this Fc domain is 35.4 kDa, consistent with the 35 kDa experimental value.
• Structural annotations (3D-structure) indicate a conserved immunoglobulin fold, likely stabilized by intrachain disulfide bonds.
• Direct protein sequencing evidence supports the exact amino acid sequence of this region.
• As a reference proteome component, it serves as a well-annotated standard in rabbit immunology.

Experimental Guidance and Technical Tips

• The immunogen corresponds exactly to the rabbit IgG Fc region (amino acids 96-323), ensuring high specificity for this constant domain in IgG heavy chains.
• This monoclonal antibody is directly conjugated to , a red fluorescent dye, and is validated for flow cytometry; confirm that your instrument’s laser and filter configuration is compatible with (similar to PE).
• For flow cytometry staining, titrate the antibody to determine the optimal working concentration for your specific sample type.
• Use an appropriate isotype control (mouse IgG1, if applicable) to assess nonspecific binding.
• Consider testing on cells expressing rabbit IgG or in assays where rabbit primary antibodies are used, ensuring no cross-reactivity with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, fluorophore conjugates, and bulk ancillary reagents. For anti-species secondary antibodies like this anti-rabbit IgG Fc mAb, CamelBio can provide customized labeling, technical consulting, and reliable supply chains to support your diagnostic and research applications.