[KO Validated] Anti-MSH2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P43246

Core Product Specifications and Parameters

Biological Background: MSH2 Function and Localization

• MSH2 is a core component of the post-replicative DNA mismatch repair (MMR) system. It forms two heterodimers: MutS alpha (with MSH6) and MutS beta (with MSH3), which recognize and bind to DNA mismatches and insertion-deletion loops (IDLs).
• MutS alpha (MSH2-MSH6) recognizes single base mismatches and dinucleotide IDLs, while MutS beta (MSH2-MSH3) handles larger IDLs up to 13 nucleotides. After binding, the heterodimer recruits MutL alpha to direct downstream repair, including strand discrimination, excision, and resynthesis.
• MSH2 recruits DNA helicase MCM9 to chromatin to unwind mismatch-containing DNA. ATP binding and hydrolysis act as a molecular switch, converting MutS alpha into a sliding clamp that diffuses along the DNA backbone. Related references: PMID:26300262
• In addition to MMR, MSH2 may participate in DNA homologous recombination repair and modulate UV-B-induced cell cycle regulation and apoptosis in melanocytes.
• MSH2 is localized in the nucleus and associates with chromosomes during DNA repair processes.
• The protein is ubiquitously expressed across human tissues and is associated with hereditary nonpolyposis colorectal cancer (HNPCC, Lynch syndrome). It functions as a tumor suppressor and undergoes post-translational modifications, including phosphorylation, acetylation, and ubiquitination.
• Alternative splicing generates multiple MSH2 isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 330–583, a conserved core domain region, which may account for the observed cross-reactivity with mouse and rat MSH2.
• For WB, a specific band at approximately 105 kDa is expected; KO validation confirms target specificity, so consider including MSH2 knockout lysate as a negative control.
• In IHC-P, nuclear staining should be anticipated; optimize antigen retrieval and antibody dilution for the tissue of interest.
• For ELISA, the antibody can serve as a capture or detection reagent; pairing with another MSH2-specific antibody may improve assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined procurement channel for IVD raw materials, including validated anti-MSH2 antibodies, matched antibody pairs for ELISA development, and bulk ancillary reagents. Our portfolio supports DNA mismatch repair research and Lynch syndrome diagnostic development, enabling seamless progression from early-stage concept to clinical application.