Anti-IL2 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P60568

Core Product Specifications and Parameters

Biological Background: IL-2 Function and Localization

• Interleukin-2 (IL-2), also known as T-cell growth factor (TCGF), is a cytokine primarily produced by activated CD4+ helper T-cells, and to a lesser extent by CD8+ T-cells and NK cells. Related references: PMID:6438535
• IL-2 binds to high-affinity (IL2RA/B/G) or low-affinity (IL2RB/G) receptor complexes, initiating JAK1/JAK3 phosphorylation and STAT5 activation to drive T-cell proliferation. Related references: PMID:16293754 PMID:16477002 PMID:7973659 PMID:8580378
• It promotes differentiation and homeostasis of effector T-cell subsets (Th1, Th2, Th17, memory CD8+) and enhances NK-cell cytolytic activity. Related references: PMID:6608729 PMID:25142963
• IL-2 is secreted and glycosylated, with structurally critical disulfide bonds, as indicated by its Uniprot annotation.
• The cytokine is essential for immune tolerance through supporting regulatory T-cell survival and proliferation.
• The IL2 gene is classified as a proto-oncogene, and chromosomal rearrangements involving this locus are associated with certain leukemias/lymphomas.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning the mature IL-2 sequence (aa 21-153); consider using recombinant human IL-2 or supernatant from activated T-cells as a positive control in WB and ELISA.
• This recombinant rabbit monoclonal antibody offers high specificity and lot-to-lot consistency, suitable for reproducible results in immunoassays.
• For WB under reducing conditions, the expected band is around 18 kDa; verify using a prestained molecular weight marker.
• For IF/ICC, fixation and permeabilization conditions should be optimized; paraformaldehyde fixation followed by Triton X-100 permeabilization may be appropriate.
• When using this antibody in ELISA, ensure that the coating antigen and detection antibody pair target non-overlapping epitopes to avoid competition.

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CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical services. For immune function studies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert assistance in sourcing rare targets such as cytokines. Our one-stop approach bridges concept to clinic, supporting your assay development needs.