Anti-HMGB2 Rabbit Monoclonal Antibody for WB - P26583

Core Product Specifications and Parameters

Biological Background: HMGB2 Function and Localization

• HMGB2 (High Mobility Group Protein B2), also known as HMG-2, is a multifunctional non-histone chromosomal protein.
• In the nucleus, HMGB2 binds DNA with a preference for non-canonical structures like single-stranded DNA, bending DNA and enhancing flexibility to facilitate transcription, chromatin remodeling, and V(D)J recombination. Related references: PMID:11909973, PMID:18413230, PMID:19522541
• It acts as a cofactor for the RAG complex in V(D)J recombination, stimulating cleavage and RAG protein binding at recombination signal sequences.
• HMGB2 localizes to the nucleus, chromosome, and cytoplasm, and can be secreted; extracellularly, it acts as a chemokine promoting proliferation and migration of endothelial cells via AGER/RAGE. Related references: PMID:19811285
• It participates in innate immunity by acting as a promiscuous DNA/RNA sensor and has antimicrobial activity in gastrointestinal epithelial tissues. Related references: PMID:23877675
• The protein is subject to acetylation, phosphorylation, and disulfide bond formation, and its activity is modulated by redox state.
• HMGB2 is expressed in gastric and intestinal tissues and is implicated in neurogenesis and articular cartilage maintenance via the LEF1/Wnt pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human HMGB2; it is advisable to confirm that the antibody recognizes the full-length protein under experimental conditions.
• For chromatin immunoprecipitation (ChIP) and CUT&Tag assays, consider optimizing crosslinking and shearing conditions as HMGB2 is a chromatin-associated protein.
• Endogenous HMGB2 is detectable in human, mouse, and rat samples; choose appropriate loading controls and validate the antibody in your specific tissue or cell line.
• For immunohistochemistry (IHC) and immunofluorescence (IF), test antigen retrieval methods to unmask epitopes in formalin-fixed paraffin-embedded sections.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a convenient one-stop sourcing service for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This HMGB2 rabbit monoclonal antibody is suitable for research into chromatin biology, transcriptional regulation, and innate immunity. For your specialized needs, CamelBio can also source rare-target antibodies and provide technical support to accelerate your diagnostic development.