Anti-Musashi-2 (MSI2) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q96DH6

Core Product Specifications and Parameters

Biological Background: Musashi-2 (MSI2) Function and Localization

• Musashi-2 (MSI2) is an RNA-binding protein that regulates target mRNA expression at the translational level.
• It plays a role in the proliferation and maintenance of stem cells, particularly in the central nervous system (by similarity).
• MSI2 is localized in the cytoplasm.
• It is ubiquitously expressed in human tissues, though at low levels.
• The protein undergoes multiple post-translational modifications, including acetylation, methylation, and phosphorylation, and is a ubiquitin-conjugated protein.
• The MSI2 gene undergoes alternative splicing, producing multiple isoforms, and has been implicated in chromosomal rearrangements.
• Structural studies (3D-structure keyword) have investigated MSI2, highlighting its RNA-binding repeat domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide (aa 200-300) of human MSI2, enabling recognition of the full-length endogenous protein.
• For Western blot (WB), a band around 35 kDa is expected; use positive controls expressing MSI2 and validate detection sensitivity in your sample system.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and primary antibody dilution to achieve specific staining; consider using known positive and negative tissue sections.
• For ELISA, immobilize the immunogen peptide or recombinant MSI2 protein and verify linearity, detection limit, and matrix compatibility.
• The antibody cross-reacts with mouse and rat MSI2 according to the supplier; confirm reactivity in the species of interest using appropriate tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research institutes, and laboratories by providing ready-to-use IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-MSI2 clone. For MSI2-related projects in stem cell biology or cancer research, we can source bulk ancillary reagents and rare targets to streamline your assay development. Partner with CamelBio to bridge your concept to clinical needs efficiently.