Anti-MSH6 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P52701

Core Product Specifications and Parameters

Biological Background: MSH6 Function and Localization

• DNA mismatch repair protein Msh6, also known as G/T mismatch-binding protein (GTBP) or p160, is a component of the post-replicative DNA mismatch repair (MMR) system.
• Heterodimerizes with MSH2 to form the MutS alpha complex, which recognizes single base mismatches and dinucleotide insertion-deletion loops (IDLs) in DNA, bending the helix and shielding ~20 base pairs upon binding.
• Upon mismatch recognition, MutS alpha recruits MutL alpha to form a ternary complex, triggering downstream repair events including strand discrimination, excision, and resynthesis.
• ATP binding and hydrolysis act as a molecular switch: mismatched DNA induces ADP→ATP exchange, converting MutS alpha into a sliding clamp that diffuses along DNA, a process essential for mismatch repair.
• Recruited to chromatin in G1 and early S phase via its PWWP domain, which specifically binds histone H3 trimethylated at Lys36 (H3K36me3), enabling early marking of replication origins.
• Localizes to the nucleus and chromosomes; mutations in MSH6 are associated with hereditary nonpolyposis colorectal cancer (HNPCC/Lynch syndrome) and other disease variants.
• Annotated with keywords: DNA damage, DNA repair, DNA-binding, ATP-binding, nucleotide-binding, host-virus interaction, and alternative splicing, reflecting its diverse regulatory roles.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide from the N-terminus, ensure that the antibody recognizes the native protein by validating in a system expressing full-length MSH6, such as HeLa or MCF7 cell lysates for WB.
• For IHC-P, optimize antigen retrieval (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and consider that MSH6 is nuclear; evaluate staining in human colorectal adenocarcinoma or tonsil tissue as positive controls.
• In IF/ICC, permeabilize cells adequately to access nuclear antigens, and consider co-staining with a nuclear marker to confirm localization.
• For ELISA, a paired antibody against a non-overlapping epitope may be required; validate with recombinant MSH6 protein or peptide competition assays.
• Because MSH6 is a large protein (153 kDa), use appropriate gel percentages (e.g., 6-8% SDS-PAGE) and extended transfer times for WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material supply chain, from early-stage research to commercial kit development. For MSH6 and other DNA repair targets, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. Partner with us to accelerate your diagnostic assay development with reliable, high-quality reagents.