Anti-MSH2 Polyclonal Antibody for WB, IF/ICC, ELISA - P43246

Core Product Specifications and Parameters

Biological Background: DNA mismatch repair protein Msh2 Function and Localization

• Core component of the post-replicative DNA mismatch repair (MMR) system. Forms two heterodimers: MutS alpha (with MSH6) and MutS beta (with MSH3), which bind mismatched DNA to initiate repair.
• MutS alpha recognizes single base mismatches and small insertion-deletion loops (1–2 nucleotides), while MutS beta recognizes larger loops up to 13 nucleotides.
• Upon binding, the heterodimers bend DNA, shield approximately 20 base pairs, and recruit MutL alpha to coordinate strand discrimination, excision, and resynthesis.
• Recruits DNA helicase MCM9 to chromatin, facilitating unwinding of the mismatch-containing strand. Related references: PMID:26300262
• ATP binding and hydrolysis act as a molecular switch: mismatched DNA triggers ADP→ATP exchange, converting MutS alpha into a sliding clamp that diffuses along DNA and enables downstream repair events.
• Also plays a role in DNA homologous recombination repair, and in melanocytes may modulate UV-B-induced cell cycle regulation and apoptosis.
• Subcellular localization is predominantly nuclear and chromosome-associated, consistent with its genomic surveillance function. Ubiquitously expressed across tissues.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination. Acts as a tumor suppressor; mutations are associated with hereditary nonpolyposis colorectal cancer (HNPCC/Lynch syndrome).

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 1–300 amino acids of human MSH2. As a polyclonal antibody, it recognizes multiple epitopes within this region, which may provide robust detection but lot-to-lot consistency should be verified.
• Expected molecular weight is 105 kDa. Suitable positive controls include commonly used human cell lines (e.g., HeLa, HEK293) and mouse/rat tissues, given the ubiquitous expression of MSH2.
• For Western blot (WB), standard protocols can be employed; consider optimizing blocking and antibody dilution for the specific sample type.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate nuclear staining. Use appropriate fixation and permeabilization methods to preserve nuclear antigens.
• The cross-reactivity with mouse and rat enables translational studies, but validation in the relevant sample system is recommended.

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CamelBio serves as a reliable one-stop partner for IVD raw-material sourcing, connecting diagnostic manufacturers and research labs with high-quality reagents like the MSH2 antibody for DNA mismatch repair and oncology applications. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities to support your entire workflow from concept to clinical application.