Anti-Moesin Monoclonal Antibody for WB, IHC-P, ELISA - P26038

Core Product Specifications and Parameters

Biological Background: Moesin Function and Localization

• Moesin (MSN) is an ERM family protein that tethers the actin cytoskeleton to the plasma membrane.
• Its activity is regulated by phosphorylation of a C-terminal threonine, which induces an open conformation for F-actin binding and cytoskeletal rearrangement. Related references: PMID:10212266
• Activated moesin controls cell shape, membrane trafficking, and signal transduction. Related references: PMID:12387735, PMID:15039356
• In the immune system, moesin is essential for lymphocyte homeostasis, self-tolerance, and egress from lymphoid organs. Related references: PMID:9298994, PMID:9616160
• It contributes to immunologic synapse formation and modulates phagolysosomal biogenesis in macrophages (By similarity). Related references: PMID:27405666
• Subcellularly, moesin is found at the cell membrane, apical microvilli, cytoplasm, and associated with the cortical cytoskeleton.
• It is expressed in all tissues and is subject to multiple post-translational modifications, including phosphorylation, acetylation, and S-nitrosylation.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 68 kDa may be observed; consider using whole-cell lysates from human, mouse, or rat samples based on cross-reactivity data.
• In immunohistochemistry (paraffin), antigen retrieval conditions may need optimization; moesin is ubiquitously expressed, so most tissues can serve as positive controls.
• For ELISA, the immunogen sequence (aa 400-500) may be used to assess direct binding; pairing with other clones is advisable for sandwich ELISA development.
• Since the antibody recognizes the C-terminal region containing the regulatory threonine, it may detect both phosphorylated and non-phosphorylated forms; validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supports diagnostic developers and research labs with an extensive portfolio of high-quality antibodies and reagents. This anti-Moesin monoclonal antibody is ideally suited for investigations into ERM family proteins, immune synapse formation, and cytoskeletal dynamics. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare or difficult targets to streamline your assay development.