Anti-Mesothelin Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q13421

Core Product Specifications and Parameters

Biological Background: Mesothelin Function and Localization

• Mesothelin, encoded by the MSLN gene, is also known as CAK1 antigen and pre-pro-megakaryocyte-potentiating factor, and undergoes alternative splicing to produce different isoforms.
• The membrane-anchored form of mesothelin may play a role in cellular adhesion, contributing to cell-cell interactions.
• The megakaryocyte-potentiating factor (MPF) domain, released after cleavage, can potentiate megakaryocyte colony formation in vitro.
• Mesothelin is localized at the cell membrane, anchored via a GPI-anchor, and also detected in the Golgi apparatus; it can be secreted after proteolytic cleavage.
• It is expressed in normal lung tissue and at low levels in heart, placenta, and kidney; in mesothelial cells, and is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas at the protein level.
• The protein undergoes several modifications, including cleavage on a pair of basic residues, N-glycosylation, disulfide bond formation, and phosphorylation.
• Key structural features include a signal peptide, a GPI-anchor, and the presence of multiple domains identified by 3D-structure studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide encompassing amino acids 500–600 of human mesothelin; this region is located in the C-terminal part of the protein. When using this antibody, consider that it may detect both the full-length precursor and the mature mesothelin or MPF fragment, depending on the sample processing.
• For Western blot (WB), anticipate a band around 69 kDa (consistent with UniProt-predicted 68 kDa) under reducing conditions. Validate the observed molecular weight in your specific cell or tissue lysates.
• In immunofluorescence of paraffin-embedded sections (IF-P), optimize antigen retrieval and permeabilization to effectively detect membrane-associated or Golgi-localized mesothelin.
• For ELISA applications, assess the antibody’s performance as a capture or detection reagent after empirical pairing tests, and validate against recombinant mesothelin or natural targets in appropriate matrices.
• Cross-reactivity is reported for human and rat; when working with rat samples, confirm specificity and sensitivity under your experimental conditions, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting to support every stage from concept to clinic. This Anti-Mesothelin Rabbit Monoclonal Antibody serves as a critical tool for mesothelin-targeted cancer research and assay development. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing solutions for rare targets to streamline your diagnostic workflows.