Anti-MSH2 Rabbit Polyclonal Antibody (KO Validated) for WB, IF/ICC, IP, ELISA - P43246

Core Product Specifications and Parameters

Biological Background: MSH2 Function and Localization

• MSH2 is a central component of the DNA mismatch repair (MMR) system, essential for maintaining genomic stability by correcting base-base mismatches and insertion-deletion loops (IDLs) that arise during DNA replication.
• It forms two heterodimers: MutSα with MSH6, which recognizes single base mismatches and small (1–2 nucleotide) IDLs, and MutSβ with MSH3, which recognizes larger IDLs up to 13 nucleotides long.
• Upon mismatch binding, the MutS heterodimer bends the DNA and shields approximately 20 base pairs, subsequently recruiting the MutLα heterodimer (MLH1-PMS2) to form a ternary complex that directs downstream repair events, including strand discrimination, excision, and resynthesis.
• MSH2 recruits DNA helicase MCM9 to chromatin, which unwinds the DNA strand containing the mismatch to facilitate repair (PubMed:26300262).
  Related references:
  PMID:26300262
• The ATPase activity of MutSα functions as a molecular switch: mismatch binding triggers ADP→ATP exchange, inducing a conformational change that converts MutSα into a sliding clamp capable of diffusing along DNA, a critical step for MMR.
• Beyond MMR, MSH2 may also participate in DNA homologous recombination repair and modulates UV-B-induced cell cycle regulation and apoptosis in melanocytes.
• MSH2 is ubiquitously expressed across tissues and localizes primarily to the nucleus and chromosomes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, and mutations in MSH2 are associated with hereditary nonpolyposis colorectal cancer (HNPCC/Lynch syndrome).

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band near 105 kDa. Include a knockout (KO) validated sample to confirm antibody specificity.
• In immunoprecipitation (IP) assays, the antibody may pull down MSH2 and its binding partners (e.g., MSH6, MSH3, MLH1). Optimize buffer conditions to preserve protein interactions.
• For immunofluorescence (IF/ICC), anticipate nuclear localization. Use appropriate fixation and permeabilization methods to ensure accessibility to the target.
• The immunogen corresponds to the N-terminal region (aa 1–300); epitopes in this region are likely well-exposed and conserved, potentially supporting cross-reactivity with rat.
• When using ELISA, titrate the antibody to determine the optimal coating concentration and detection range; consider a direct or sandwich format depending on assay design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete sourcing bridge for IVD raw materials, supporting your transition from research to diagnostics. With this MSH2 antibody targeting the DNA mismatch repair pathway, we can also help you secure validated antibody pairs, bulk ancillary reagents, and custom rare-target materials to streamline your assay development in cancer research.