Anti-MUC2 Monoclonal Antibody for IF-P, IHC-P, ELISA - Q02817

Core Product Specifications and Parameters

Biological Background: MUC2 Function and Localization

• Coats intestinal epithelia, forming a protective lubricating barrier against particles and infectious agents. Related references: PMID:17058067, PMID:19432394, PMID:33031746
• Major constituent of colon mucus; forms polymeric networks that act as a hydrogel, guarding the epithelium from pathogens while allowing nutrient absorption and gas exchange. Related references: PMID:19432394, PMID:33031746, PMID:36206754
• Functions as a divalent copper chaperone, binding Cu(2+) and Cu(1+) to protect intestinal cells from copper toxicity and facilitate nutritional copper uptake. Related references: PMID:36206754
• Goblet cells synthesize two MUC2 forms: a 'thick' proximally produced mucus that wraps microbiota, and a 'thin' distally produced lubricating mucus (By similarity).
• Mucin gels store antimicrobial molecules, contributing to innate immunity, and also house and feed the gut microbiome. Related references: PMID:33031746
• Subcellular location: Secreted.
• Tissue specificity: Expressed in colon, small intestine, colonic tumors, bronchus, cervix, and gall bladder.
• Key structural features include autocatalytic cleavage, calcium and copper binding, disulfide bonds, and extensive O-glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 858-1259, a region rich in potential O-glycosylation sites; consider this when designing antigen retrieval or blocking steps for IHC.
• For IHC-P, MUC2 is highly expressed in goblet cells of colon and small intestine; use appropriate positive control tissues (e.g., human colon sections) and consider mucin-specific fixation/permeabilization protocols to preserve the mucus gel.
• For IF-P, the large size of MUC2 may require extended permeabilization; antigen retrieval with mild denaturants may improve detection.
• For ELISA, the recombinant immunogen may be used as a positive control; validate assay conditions with native mucin preparations if absolute quantitation is needed.
• Cross-reactivity with mouse and rat is confirmed; validate in the relevant species' intestinal tissues to ensure specificity.
• The high molecular weight (~551 kDa) may cause challenges in standard Western blotting; consider using low-percentage agarose gels or dot blot formats if exploring alternative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, supporting mucin and gastrointestinal disease research with high-quality antibody reagents. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source raw materials for rare targets like MUC2 to accelerate your diagnostic and research workflows. Let us bridge your needs from concept to clinic.