Anti-MTHFD2 Rabbit Monoclonal Antibody for WB, ELISA - P13995

Core Product Specifications and Parameters

Biological Background: MTHFD2 Function and Localization

• MTHFD2 encodes a bifunctional mitochondrial enzyme with methylenetetrahydrofolate dehydrogenase and cyclohydrolase activities.
• It functions in one-carbon metabolism, and its dehydrogenase activity is NAD-specific but can utilize NADP at reduced efficiency.
• The enzyme localizes to the mitochondrion, as indicated by a transit peptide for mitochondrial import.
• Post-translational modifications include acetylation and ubiquitination.
• The protein requires magnesium as a cofactor and is classified as a multifunctional enzyme.
• Alternative splicing results in multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 40-250, an internal region, suggesting detection of both full-length and possibly truncated forms; use in WB with appropriate loading controls.
• For WB, a band at ~38 kDa is expected; validate specificity in human and mouse cell lysates as per cross-reactivity data.
• For ELISA, standard indirect or sandwich ELISA protocols may be used; optimization of coating antigen and antibody concentration is recommended.
• Consider testing in other species if required, based on sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers with one-stop access to IVD raw materials, including this MTHFD2 rabbit monoclonal antibody for research on one-carbon metabolism targets. Our portfolio covers validated antibody pairs, bulk ancillary reagents, and rare target sourcing, backed by technical consulting from concept to clinic.