Anti-Myosin Heavy Chain Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - P12883 / P13533

Core Product Specifications and Parameters

Biological Background: Myosin Heavy Chain Function and Localization

• Myosin-7, encoded by the MYH7 gene, is a class II myosin heavy chain also known as the beta-cardiac myosin isoform (MyHC-β) or slow skeletal muscle isoform (MyHC-slow). It is a major component of the thick filaments in cardiac and slow-twitch skeletal muscle.
• Myosins are actin-based motor molecules with intrinsic ATPase activity, converting chemical energy from ATP hydrolysis into mechanical force for muscle contraction. Myosin-7 specifically drives sarcomere shortening by cyclically interacting with actin thin filaments.
• Myosin-7 assembles into bipolar thick filaments via its coiled-coil tail domain, which together with actin thin filaments forms the fundamental contractile unit of striated muscle.
• Subcellularly, Myosin-7 localizes to the cytoplasm, particularly within myofibrils and the A-band of sarcomeres, where thick filaments are anchored.
• At the protein level, both wild-type and certain disease-linked variants (e.g., Gln-403) are detected in skeletal muscle, indicating expression beyond cardiac tissue.
• Post-translational modifications include methylation and phosphorylation, which may regulate motor function and filament assembly. Myosin-7 is also associated with cardiomyopathy when mutated, highlighting its critical role in cardiac muscle physiology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the rod/tail domain (aa 1200–1400) of human Myosin heavy chain (P12883), a region likely conserved across species. This may explain the observed cross-reactivity with mouse and rat.
• For western blotting, Myosin heavy chain runs at approximately 224 kDa. Consider using low-percentage gels (e.g., 6% or gradient) and extended transfer times for efficient high-molecular-weight protein transfer.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimal antigen retrieval and permeabilization conditions should be empirically determined. Include appropriate positive and negative controls to confirm specificity.
• For ELISA applications, the antibody may be used as a capture or detection reagent. Pairing with a compatible secondary antibody or detection system should be optimized for the specific assay format.
• As a rabbit monoclonal antibody, it provides consistent batch-to-batch reproducibility and high affinity, suitable for quantitative assays and long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing bridge for IVD raw materials, providing diagnostic manufacturers and research labs with reliable access to premium antibodies like this anti-Myosin heavy chain clone, ideal for cardiac and skeletal muscle biomarker development. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to support your complete assay development workflow—from concept to clinic.