Anti-Musashi-2 (MSI2) Rabbit Polyclonal Antibody for WB, ELISA - Q96DH6

Core Product Specifications and Parameters

Biological Background: Musashi-2 Function and Localization

• RNA-binding protein Musashi homolog 2 (MSI2) is encoded by the MSI2 gene and functions as an RNA-binding protein that regulates target mRNA expression at the translation level.
• MSI2 is implicated in the proliferation and maintenance of stem cells, particularly within the central nervous system (by similarity).
• The protein localizes predominantly to the cytoplasm, consistent with its role in post-transcriptional regulation.
• Tissue expression is ubiquitous but generally low across various human tissues, as indicated by detection at low levels.
• Post-translational modifications of MSI2 include acetylation, methylation, phosphorylation, and ubiquitination (Ubl conjugation), which may modulate its activity and stability.
• The protein features RNA recognition motifs (RRMs) and contains repeat regions critical for RNA binding.
• Alternative splicing generates multiple transcript variants, and MSI2 has been associated with chromosomal rearrangements in certain disease contexts.
• Keywords associated with MSI2 in UniProt include: 3D-structure, Acetylation, Alternative splicing, Chromosomal rearrangement, Cytoplasm, Methylation, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, RNA-binding, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody corresponds to amino acids 1–80 of human Musashi-2 (NP_620412.1). Researchers should verify reactivity if the target region is mutated or absent in specific isoforms or experimental models.
• For western blotting, the expected molecular weight is approximately 35 kDa. Note that post-translational modifications may cause slight shifts in band migration; optimize gel percentage and transfer conditions accordingly.
• When performing ELISA, titrate the antibody and antigen to determine optimal working concentrations, and include appropriate positive and negative controls.
• Validate cross-reactivity in your specific sample matrix, as this antibody is reported to recognize both human and mouse MSI2.
• Store the antibody at recommended temperatures (shipment on ice bags) and avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research laboratories, offering one-stop access to IVD raw materials from concept to clinic. This anti-Musashi-2 rabbit polyclonal antibody exemplifies our capability to supply validated antibodies for stem cell and neuroscience research. Beyond individual antibodies, we provide optimized antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and scalable production needs.