[KO Validated] Anti-SHMT2 Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - P34897

Core Product Specifications and Parameters

Biological Background: SHMT2 Function and Localization

• SHMT2 (Serine Hydroxymethyltransferase 2) is a mitochondrial enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate, a critical one‑carbon donor for purine biosynthesis. Related references: PMID:24075985 PMID:25619277 PMID:29364879
• Through its role in glycine and folate metabolism, SHMT2 contributes to the de novo mitochondrial thymidylate biosynthesis pathway, preventing uracil accumulation in mitochondrial DNA. Related references: PMID:21876188
• SHMT2 is required for mitochondrial translation by producing 5,10‑methylenetetrahydrofolate, which provides the methyl donor for taurinomethyluridine modification at the wobble position of certain mitochondrial tRNAs. Related references: PMID:29364879 PMID:29452640
• The protein associates with mitochondrial DNA and is found in the mitochondrial nucleoid. Related references: PMID:18063578
• Beyond its mitochondrial roles, SHMT2 acts as a component of the BRISC complex and participates in IFNAR1 deubiquitination, influencing innate immune signaling. Related references: PMID:24075985
• SHMT2 localizes predominantly to the mitochondrial matrix, nucleoid, and inner membrane, but is also detected in the cytoplasm and nucleus under certain conditions.
• Post‑translational modifications include phosphorylation and acetylation; it is a pyridoxal phosphate‑dependent enzyme and belongs to the transferase family.
• Genetic variants in SHMT2 are associated with diseases such as intellectual disability and cardiomyopathy.

Experimental Guidance and Technical Tips

• The immunogen was designed from the C‑terminal region (aa 405–504) of human SHMT2; this region is well conserved across human, mouse, and rat, supporting the reported cross‑reactivity.
• For Western blotting, SHMT2 may be detected at 53–56 kDa depending on isoform and modification status; pre‑stained molecular weight markers are recommended for accurate sizing.
• In immunofluorescence and immunohistochemistry, consider validating the detection of mitochondrial SHMT2 by co‑staining with a mitochondrial marker, as the protein can also shuttle to the nucleus/cytoplasm.
• ELISA performance should be empirically determined; direct and sandwich formats can be explored with appropriate antigen and detection conditions.
• This antibody is reported as KO validated; researchers should perform their own knockout or knockdown confirmation in the relevant cell line to verify specificity for critical publications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in connecting diagnostic manufacturers and research laboratories with premium IVD raw materials, offering this anti‑SHMT2 antibody as part of a comprehensive portfolio for one‑carbon metabolism and mitochondrial disease studies. Our supply network covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to support your development from early‑stage research to clinical application. For tailored procurement solutions and technical consultation, please contact our team.