Anti-IDH2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P48735

Core Product Specifications and Parameters

Biological Background: IDH2 Function and Localization

• IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that plays a critical role in intermediary metabolism and energy production (PubMed:19228619, PubMed:22416140). Related references: PMID:19228619 PMID:22416140
• The enzyme may tightly associate or interact with the pyruvate dehydrogenase complex, linking TCA cycle activity to other metabolic pathways (PubMed:19228619, PubMed:22416140). Related references: PMID:19228619 PMID:22416140
• Alternative splicing of IDH2 produces multiple isoforms, potentially diversifying its function.
• Located in the mitochondrion, where it participates in the tricarboxylic acid cycle (keyword: Tricarboxylic acid cycle; subcellular location: Mitochondrion).
• Keywords also indicate involvement in the glyoxylate bypass and metal-binding (magnesium, manganese), reflecting its catalytic mechanism.
• Post-translational modifications include acetylation, which may regulate activity.
• Disease-associated variants of IDH2 have been reported, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 353–452 of human IDH2; this C-terminal region should be considered for epitope mapping studies.
• For western blotting, a band near 51 kDa is expected; use a positive control lysate from human, mouse, or rat tissues expressing IDH2.
• In immunohistochemistry, validate the antibody in relevant tissue sections known to express IDH2.
• For immunofluorescence, confirm mitochondrial localization using appropriate counterstains.
• ELISA applications may require optimization of coating antigen and blocking conditions.
• Cross-reactivity with mouse and rat suggests conserved epitopes; verify performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide one-stop access to high-quality IVD raw materials. This IDH2 Rabbit Monoclonal Antibody, targeting a key mitochondrial enzyme, is ideal for metabolism and disease mechanistic studies. Beyond validated antibodies, we offer antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate your clinical development.