ENPP2 Rabbit pAb for WB, ELISA - Q13822

Core Product Specifications and Parameters

Biological Background: ENPP2 Function and Localization

• ENPP2, also known as Autotaxin, is an ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2) with lysophospholipase D activity.
• It hydrolyzes lysophosphatidylcholine to produce lysophosphatidic acid (LPA), a potent signaling molecule involved in cell motility, angiogenesis, and neurite outgrowth. Related references: PMID:12354767, PMID:14500380, PMID:15769751
• ENPP2 can also act on sphingosylphosphorylcholine to generate sphingosine-1-phosphate, modulating cell migration. Related references: PMID:14500380
• The enzyme is secreted and found in various body fluids including plasma, CSF, and follicular fluid, aligning with its extracellular role in LPA production.
• It is predominantly expressed in brain, placenta, ovary, and small intestine, and upregulated in multiple carcinomas.
• ENPP2 is a glycoprotein with a signal peptide, disulfide bonds, and zinc-binding sites; it undergoes cleavage on pairs of basic residues.
• The protein plays a role in platelet activation by cleaving sn-1 lysophospholipids to generate LPA with specific fatty acid chains. Related references: PMID:21393252
• It is involved in pathophysiological processes such as obesity and cancer, as indicated by its association with cell proliferation and adipose tissue development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 564-863) of human ENPP2, suggesting recognition of both full-length and potential C-terminal isoforms.
• For Western blotting, a band near 99 kDa is expected; consider using reducing conditions and validate with positive controls (e.g., cell lines expressing ENPP2).
• For ELISA applications, pairing with an appropriate capture or detection antibody may be optimized; the polyclonal nature can provide broad epitope coverage for sensitive detection.
• Given the predicted secretion, consider testing cell supernatants or body fluids for target detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop source for IVD raw materials, offering diagnostic manufacturers and researchers streamlined access to validated antibodies, optimized pairs, and ancillary reagents. For ENPP2/Autotaxin studies in cancer biology and lipid signaling, CamelBio can supply high-quality polyclonal antibodies and assist with custom or bulk-order requirements, accelerating your R&D pipeline from concept to clinic.