Anti-RheB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15382

Core Product Specifications and Parameters

Biological Background: RheB Function and Localization

• GTP-binding protein Rheb (Ras homolog enriched in brain), also known as RHEB2, is a small GTPase that functions as an allosteric activator of mTORC1 complex (PubMed:12172553, PubMed:12271141, PubMed:12842888). Related references: PMID:12172553, PMID:12271141, PMID:12842888
• In response to nutrients, growth factors, or amino acids, Rheb activates mTOR by inducing a conformational change that enhances phosphorylation of downstream targets RPS6KB1/S6K1 and EIF4EBP1/4E-BP1 (PubMed:29236692, PubMed:33157014). Related references: PMID:29236692, PMID:33157014
• Rheb is required for lysosomal localization of the TSC-TBC complex; under starvation, it is inactivated by TSC-TBC to prevent mTORC1 activation (PubMed:24529379, PubMed:33157014). Related references: PMID:24529379, PMID:33157014
• It possesses low intrinsic GTPase activity (PubMed:15340059). Related references: PMID:15340059
• Subcellularly, Rheb localizes to endomembrane system including lysosome, Golgi, and ER membranes, as well as cytosol.
• Tissue expression is ubiquitous, with highest levels in skeletal and cardiac muscle (PubMed:8543055).
• Post-translational modifications include prenylation (lipoprotein), methylation, phosphorylation, and isopeptide bond formation (Ubl conjugation).

Experimental Guidance and Technical Tips

• For Western blot, a band around 20 kDa is expected; consider using positive controls from human, mouse, or rat cell lysates, particularly those with high RheB expression (e.g., muscle tissues).
• In immunocytochemistry, validate fixation and permeabilization protocols; RheB localizes to endomembrane structures, resulting in punctate staining.
• For ELISA, direct or sandwich formats may be explored; cross-reactivity with mouse and rat offers flexibility in multi-species assay development.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; pre-adsorption steps may reduce non-specific binding if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, recombinant proteins, and bulk reagents to streamline diagnostic development. This RheB polyclonal antibody supports mTOR pathway research and biomarker assay establishment. Complement your workflow with matched ancillary materials and expert technical assistance from concept to clinic.