Anti-Nephrin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9QZS7

Core Product Specifications and Parameters

Biological Background: Nephrin Function and Localization

• Nephrin (encoded by Nphs1) is a transmembrane adhesion receptor of the immunoglobulin superfamily, with multiple isoforms generated by alternative splicing; isoform 1 is the predominant form in adult kidney.
• It plays a critical role in the development and function of the kidney glomerular filtration barrier, regulating vascular permeability and anchoring the podocyte slit diaphragm to the actin cytoskeleton.
• Nephrin is also involved in skeletal muscle formation by regulating myoblast fusion, and is expressed in muscle during embryonic development and following acute injury.
• The protein is localized at the cell membrane, consistent with its single-pass transmembrane structure.
• Highly expressed in kidney glomerular podocytes; additionally detected in developing cerebellum, hindbrain, spinal cord, retina, hypothalamus, and skeletal muscle during myoblast fusion. Isoforms 1 and 2 are expressed in newborn brain and developing cerebellum.
• Nephrin undergoes N-linked glycosylation, contains disulfide bonds, and is phosphorylated; it is classified as a developmental protein with immunoglobulin-like repeats.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to the C-terminal cytoplasmic region (aa 1033-1256) of mouse Nephrin, recognizing an intracellular epitope.
• For immunofluorescence on paraffin sections (IF-P) or immunohistochemistry (IHC-P), consider including a permeabilization step to allow access to the intracellular domain, and validate in appropriate mouse tissue samples.
• In western blotting (WB), Nephrin is detected as a band of approximately 135–136 kDa in mouse kidney or skeletal muscle lysates; ensure proper sample preparation to preserve membrane protein integrity.
• As a rabbit monoclonal antibody, it offers high batch-to-batch consistency and is suitable for quantitative ELISA applications.
• Cross-reactivity with other species has not been tested; for non-mouse experiments, conduct species verification if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated procurement partner for IVD raw materials, offering one-stop access to premium antibodies, antigens, and ancillary reagents. The Nephrin Rabbit Monoclonal Antibody addresses nephropathy and podocyte biology research, complementing our portfolio of renal disease markers. Beyond standard catalog items, we provide customized bulk supply solutions—including validated antibody pairs and rare target sourcing—to support your assay development from concept to clinical application.