Anti-CD304/Neuropilin-1 Rabbit PolymAb® for WB, IF/ICC, IHC-P, ELISA - P97333

Core Product Specifications and Parameters

Biological Background: CD304/Neuropilin-1 Function and Localization

• Neuropilin-1 (NRP1, also known as A5 protein) is a transmembrane receptor encoded by the Nrp1 gene, expressed predominantly in the nervous system.
• It plays essential roles in cardiovascular development, angiogenesis, and neuronal circuit formation, as well as organogenesis outside the nervous system.
• NRP1 mediates chemorepulsive axon guidance by binding semaphorin 3A (SEMA3A). Related references: PMID:26503042
• Binding to VEGFA triggers signaling required for motor neuron axon guidance and cell body migration, e.g., facial motor neuron migration during embryogenesis. Related references: PMID:26503042
• Recognizes C-end rule (CendR) motifs (R/KXXR/K) on ligands, causing cellular internalization and vascular leakage.
• Binds growth factors including VEGF165, VEGFB, and PLGF-2; co-receptors with KDR to enhance VEGF signaling and chemotaxis.
• Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.
• Subcellularly localizes to the mitochondrial membrane, cell membrane, and cytoplasm.
• Keywords: angiogenesis, neurogenesis, differentiation, receptor, glycoprotein, heparin-binding, and others.

Experimental Guidance and Technical Tips

• The immunogen is recombinant full-length mouse CD304/Neuropilin-1 protein; the antibody may recognize native and denatured forms depending on the application.
• For Western blot, a band of approximately 103 kDa is predicted; validate with appropriate positive controls (e.g., mouse brain or endothelial cell lysates).
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), consider optimizing fixation and antigen retrieval protocols to preserve epitope accessibility.
• Due to reported cross-reactivity with human and rat NRP1, this antibody may be suitable for multi-species studies; always confirm specificity in the relevant system.
• For ELISA, preliminary titration and sandwich pair testing are recommended before use in quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated antibodies like this Neuropilin-1 Rabbit PolymAb®, alongside bulk ancillary reagents and custom sourcing for rare targets. Whether you are developing angiogenesis assays, neurological diagnostics, or cardiovascular tests, our one-stop platform accelerates your path from concept to clinic with reliable raw-material supply.