Anti-Enpp1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - G3X9S2

Core Product Specifications and Parameters

Biological Background: Enpp1 Function and Localization

• Protein identity: Enpp1 encodes ectonucleotide pyrophosphatase/phosphodiesterase family member 1, also known as alkaline phosphodiesterase I, nucleotide pyrophosphatase, nucleotide diphosphatase, phosphodiesterase I/nucleotide pyrophosphatase 1, and plasma-cell membrane glycoprotein PC-1 (PC-1).
• Enzymatic classification: It is a hydrolase and phosphodiesterase, suggesting a role in the cleavage of phosphodiester and pyrophosphate bonds in extracellular nucleotides.
• Subcellular localization: Primarily localized to the basolateral cell membrane and also secreted into the extracellular milieu via a signal-anchor mechanism.
• Membrane topology: A single-pass type II transmembrane protein that forms disulfide-linked homodimers.
• Cofactors: Binds zinc and calcium ions, indicative of metalloenzyme activity.
• Post-translational modifications: Glycoprotein and phosphoprotein, which may influence its stability, trafficking, or enzymatic activity.
• Biomineralization: Associated with biomineralization processes, likely through regulation of extracellular pyrophosphate levels.
• Reference proteome: Serves as the reference proteome for this gene product, representing the canonical sequence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 580-730) of mouse Enpp1, and the antibody may recognize similar epitopes in human and rat due to cross-reactivity.
• For Western blot (WB), a band near the predicted molecular weight of ~103 kDa may be observed; consider that glycosylation and other modifications can influence migration.
• For immunofluorescence/immunocytochemistry (IF/ICC), the protein is located at the cell membrane and is also secreted; permeabilization may be required for intracellular detection, while live-cell staining can capture surface pools.
• ELISA performance can be verified in the relevant sample system, with optimization of coating and detection conditions.

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