Anti-EN2 Rabbit Polyclonal Antibody for WB, ELISA - P19622

Core Product Specifications and Parameters

Biological Background: EN2 Function and Localization

• EN2 is a homeobox-containing protein, suggesting a role as a DNA-binding transcription factor.
• It is annotated as a developmental protein, indicating involvement in developmental processes.
• The protein localizes predominantly to the nucleus, consistent with its function as a transcription regulator.
• EN2 has been implicated in autism spectrum disorders, as reflected by UniProtKB keywords.
• It is part of the human reference proteome and has been identified in proteomics analyses.
• The predicted molecular weight of EN2 is approximately 34 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–333 in the C-terminal region of human EN2. This region may be conserved across species, as the antibody cross-reacts with mouse and rat EN2.
• For Western blotting, a specific band at ~34 kDa is expected. Include appropriate positive and negative controls to confirm antibody specificity.
• For ELISA, consider using the immunogen peptide as a coating antigen or positive control.
• Optimize antibody dilution and incubation conditions for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to support your EN2-related studies in developmental biology and autism research.