Anti-AEBP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IUX7

Core Product Specifications and Parameters

Biological Background: AEBP1 Function and Localization

• AEBP1, also known as aortic carboxypeptidase-like protein (ACLP), is a multifunctional protein with roles in extracellular matrix organization, transcriptional regulation, and metabolic signaling.
• Acts as a positive regulator of collagen fibrillogenesis, playing a key role in the organization and remodeling of the extracellular matrix.
• In adipocytes, enhances MAP-kinase activity, promoting proliferation and reducing differentiation, thereby influencing adipose tissue dynamics.
• Positively regulates NF-kappa-B activity in macrophages by facilitating phosphorylation and degradation of I-kappa-B-alpha, which enhances inflammatory responsiveness.
• Functions as a transcriptional repressor, indicating its involvement in gene expression control within the nucleus.
• Localizes to the secreted compartment, cytoplasm, and nucleus, reflecting its diverse functional sites.
• Specifically expressed in osteoblasts and visceral fat, with broader detection in proteomics studies.
• Contains calmodulin-binding and DNA-binding domains, and mutations are linked to Ehlers-Danlos syndrome (glycoprotein, alternative splicing, disease variant).

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 999-1158), which may target conserved epitopes and support the observed cross-reactivity with mouse and rat.
• For Western blot, expect a band at approximately 131 kDa; include appropriate molecular weight markers and loading controls for accurate size estimation.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization to detect AEBP1 in its cytoplasmic and nuclear locations.
• For ELISA applications, perform antibody titration to determine optimal working dilutions for quantitative detection.
• Validate the antibody in the relevant sample system (e.g., human, mouse, or rat cell lysates or tissue sections) to confirm specificity and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering a comprehensive portfolio from concept to clinic. This AEBP1 antibody supports research in extracellular matrix biology, inflammatory pathways, and metabolic disorders. Beyond antibodies, we provide validated antibody pairs, bulk ancillary reagents, and customized raw-material sourcing to accelerate your diagnostic development.