SNRPN Rabbit Polyclonal Antibody for WB and ELISA - P63162

Core Product Specifications and Parameters

Biological Background: SNRPN Function and Localization

• SNRPN encodes the small nuclear ribonucleoprotein-associated protein N (Sm-N), a core component of the spliceosomal Sm protein family.
• It is also known as Sm protein D (Sm-D) and tissue-specific-splicing protein, reflecting its homology to canonical Sm proteins.
• The protein is predicted to play a role in tissue-specific alternative RNA processing events, influencing mRNA splicing regulation.
• SNRPN is predominantly localized in the nucleus, consistent with its involvement in spliceosomal complexes.
• Expression is enriched in brain and lymphoblast tissues, indicating a specialized function in these cell types.
• Key post-translational modifications include methylation, and the protein features repetitive sequences and RNA-binding domains.
• Several isoforms exist due to alternative splicing, contributing to functional diversity.
• SNRPN is part of the ribonucleoprotein complex, critical for pre-mRNA splicing.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide from the N-terminal region (aa 1–100); consider sequence alignment with mouse/rat homologs to predict cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 25 kDa; validate in relevant tissue or cell lysates with appropriate controls.
• In ELISA, optimize coating conditions and antibody dilution; inclusion of blocking steps can reduce non-specific binding.
• As a polyclonal antibody, confirm lot-to-lot consistency; use positive (e.g., brain lysate) and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For nuclear splicing targets like SNRPN, CamelBio provides end-to-end sourcing support to accelerate diagnostic assay development from concept to clinic.