Anti-RALY Rabbit Polyclonal Antibody for WB, ELISA - Q9UKM9

Core Product Specifications and Parameters

Biological Background: RALY Function and Localization

• RALY (RNA-binding protein Raly), also known as autoantigen p542 or heterogeneous nuclear ribonucleoprotein C-like 2 (hnRNP C-like 2), is encoded by the RALY gene (aliases HNRPCL2, P542).
• RALY functions as a transcriptional cofactor for cholesterol biosynthetic genes in the liver by binding the lipid-responsive non-coding RNA LeXis (By similarity).
• It may also act as a heterogeneous nuclear ribonucleoprotein (hnRNP), suggesting a role in mRNA processing and splicing. Related references: PMID:9376072
• The protein localizes predominantly to the nucleus.
• RALY is widely expressed, with detection in heart, brain, lung, liver, skeletal muscle, kidney, and pancreas, and weaker expression in placenta.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, pointing to complex regulation (based on UniProt keywords).
• Alternative splicing generates multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 96–306 of human RALY; reactivity with endogenous protein in human, mouse, and rat samples is indicated, but users should validate species-specific detection in their experimental systems.
• For Western blot, the expected molecular weight is approximately 32 kDa. Include appropriate positive and negative controls, and consider using nuclear extracts to enrich the target.
• This polyclonal antibody is suitable for ELISA applications; for sandwich ELISA, ensure proper antibody pairing and validate sensitivity with recombinant protein or cell lysates.
• Since RALY is a nuclear RNA-binding protein, sample preparation and lysis protocols should consider nuclear compartment extraction to improve detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers with validated antibodies and supporting reagents from early development to production. This RALY antibody is positioned for applications in cholesterol metabolism and RNA processing research areas, and CamelBio can also assist in sourcing complementary reagents such as detection antibodies, recombinant proteins, and custom formulations. For rare target raw-material sourcing or bulk requirements, our team provides tailored procurement solutions to accelerate your assay development pipeline.