Anti-RAB2A Rabbit Polyclonal Antibody for WB and ELISA - P61019

Core Product Specifications and Parameters

Biological Background: RAB2A Function and Localization

• RAB2A is a small GTPase of the Rab family, cycling between active GTP-bound and inactive GDP-bound states, and serves as a key regulator of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Related references: PMID:37821429
• It regulates autophagy by promoting autophagosome-lysosome fusion through recruitment of the HOPS endosomal tethering complex; autophagosomal RAB2A and lysosomal RAB39A cooperatively recruit HOPS subcomplexes to mediate membrane tethering and SNARE-driven fusion. Related references: PMID:37821429
• Required for protein transport from the endoplasmic reticulum to the Golgi complex and maintains the compacted morphology of the Golgi apparatus. Related references: PMID:26209634
• Together with RAB2B, redundantly required for efficient autophagic flux. Related references: PMID:28483915
• Localizes to the endoplasmic reticulum-Golgi intermediate compartment, melanosomes, endoplasmic reticulum membrane, Golgi apparatus membrane, cytoplasmic vesicles, secretory vesicles including the acrosome, and autophagosome membranes.
• The protein undergoes prenylation for membrane association and is subject to acetylation and alternative splicing, as indicated by proteomics and structural studies.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human RAB2A (aa 1–212); verify sequence identity across species if cross-reactivity is expected beyond human, mouse, and rat.
• For Western blot, an expected band around 24 kDa may be observed; include appropriate positive controls (e.g., human, mouse, or rat cell lysates) and consider the possibility of post-translational modifications or isoforms.
• For ELISA, the recombinant immunogen can be used as a coating antigen or reference standard; optimize coating and detection conditions empirically.
• Antibody performance should be validated in the relevant sample system and application, as endogenous expression levels and protein interactions may vary.
• Given RAB2A’s role in autophagy and trafficking, functional assays may require careful consideration of cellular stimuli or inhibitors to modulate Rab activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to a comprehensive portfolio of IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For RAB2A-related projects, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether scaling up a diagnostic kit or exploring autophagy and membrane trafficking pathways, CamelBio streamlines your procurement with reliable supply and expert guidance.