Anti-ANPRB/NPR2 Rabbit Monoclonal Antibody for WB, ELISA - P20594

Core Product Specifications and Parameters

Biological Background: ANPRB/NPR2 Function and Localization

• ANPRB/NPR2 (Atrial natriuretic peptide receptor 2), also known as GC-B, is a transmembrane guanylyl cyclase receptor belonging to the natriuretic peptide receptor family.
• It serves as the primary receptor for C-type natriuretic peptide (CNP), and upon ligand binding, catalyzes the synthesis of the second messenger cGMP from GTP.
• This signaling pathway is implicated in various physiological processes, notably the regulation of skeletal growth and endochondral ossification.
• Subcellularly, the receptor is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• ANPRB/NPR2 is subject to post-translational modifications including glycosylation and phosphorylation, and contains disulfide bonds typical of receptor extracellular domains.
• Alternative splicing of the NPR2 gene produces multiple isoforms, and mutations in this gene are associated with diseases such as acromesomelic dysplasia, Maroteaux type.

Experimental Guidance and Technical Tips

• The immunogen was designed from a peptide within the intracellular region (aa 800-900) of human ANPRB/NPR2, which is highly conserved; cross-reactivity with mouse is reported.
• For Western blot, the predicted molecular weight is ~117 kDa; ensure optimal gel percentage and transfer conditions for high molecular weight proteins.
• This antibody may be suitable for detecting both glycosylated and unglycosylated forms; consider using deglycosylation enzymes if band shifts are observed.
• ELISA applications may require optimization of coating antigen and detection system; validate with known CNP-stimulated samples to confirm functional receptor detection.
• Since the receptor is membrane-bound, consider using membrane protein extraction methods for enriched detection in tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of IVD raw materials, from concept to clinic. For investigations into natriuretic peptide signaling and skeletal growth regulation, we offer this rabbit monoclonal antibody against ANPRB/NPR2 alongside validated antibody pairs, ancillary reagents, and sourcing support for rare targets. Our integrated supply chain ensures consistent quality and scalability for your assay development needs.