Polyclonal Antibodies
Anti-NRAS Rabbit Polyclonal Antibody for WB - P01111
Item Number : CM0028193
Price varies based on specs and customizations
- Application
- WB, IF/ICC, ELISA
- Cross Reactivity
- Human, Mouse, Rat
- Protein Weight
- 21kDa
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Core Product Specifications and Parameters
| Parameter | Value |
|---|---|
| Product Name | NRAS Rabbit pAb |
| Remarks/Alias | NS6; CMNS; KRAS; NCMS; ALPS4; N-ras; NRAS1; NRAS |
| Species | Human |
| Gene ID (Human) | 4893 |
| Gene ID | 4893 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 90-189 of human NRAS (NP_002515.1). |
| Source | Rabbit |
| Category | Polyclonal Antibodies |
| Application | WB, IF/ICC, ELISA |
| Cross Reactivity | Human, Mouse, Rat |
| SWISS | P01111 |
| Protein Weight | 21kDa |
| Shipping | Ice bag |
Biological Background: NRAS Function and Localization
- NRAS is a member of the Ras small GTPase family, also known as Transforming protein N-Ras (gene synonym HRAS1), with aliases NS6, CMNS, KRAS, NCMS, ALPS4, N-ras, and NRAS1.
- It functions as a signal transducer in the Ras-MAPK signaling pathway, regulating cell proliferation and survival. Related references: PMID:30712867
- NRAS possesses intrinsic GTPase activity, cycling between an active GTP-bound and inactive GDP-bound state. Related references: PMID:30712867
- The protein is recognized by LZTR1, which mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. Related references: PMID:40934300
- Subcellularly, NRAS is localized to the cell membrane and the Golgi apparatus membrane.
- Post-translational modifications include acetylation, methylation, palmitoylation, prenylation, phosphorylation, and isopeptide bond formation, as indicated by UniProt keywords.
- NRAS is classified as a proto-oncogene; mutations are associated with various cancers (keyword: Disease variant).
Experimental Guidance and Technical Tips
- For Western blot, NRAS is predicted at ~21 kDa; however, prenylation and membrane association may cause shifts. Validate the band size in your specific sample system.
- In immunofluorescence/immunocytochemistry, expect cell membrane and Golgi apparatus staining. Co-localization studies with compartment markers are recommended.
- The immunogen sequence (amino acids 90–189) is conserved across human, mouse, and rat, supporting cross-reactivity in these species; still, verify reactivity in your experimental model.
CamelBio: Your One-Stop Sourcing Bridge
CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibodies like this NRAS polyclonal antibody, along with bulk ancillary reagents and custom rare-target monoclonal antibody development. Whether advancing oncology diagnostics or research, we bridge your supply needs from concept to clinic.
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