Anti-NRAS Rabbit Polyclonal Antibody for WB - P01111

Core Product Specifications and Parameters

Biological Background: NRAS Function and Localization

• NRAS is a member of the Ras small GTPase family, also known as Transforming protein N-Ras (gene synonym HRAS1), with aliases NS6, CMNS, KRAS, NCMS, ALPS4, N-ras, and NRAS1.
• It functions as a signal transducer in the Ras-MAPK signaling pathway, regulating cell proliferation and survival. Related references: PMID:30712867
• NRAS possesses intrinsic GTPase activity, cycling between an active GTP-bound and inactive GDP-bound state. Related references: PMID:30712867
• The protein is recognized by LZTR1, which mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. Related references: PMID:40934300
• Subcellularly, NRAS is localized to the cell membrane and the Golgi apparatus membrane.
• Post-translational modifications include acetylation, methylation, palmitoylation, prenylation, phosphorylation, and isopeptide bond formation, as indicated by UniProt keywords.
• NRAS is classified as a proto-oncogene; mutations are associated with various cancers (keyword: Disease variant).

Experimental Guidance and Technical Tips

• For Western blot, NRAS is predicted at ~21 kDa; however, prenylation and membrane association may cause shifts. Validate the band size in your specific sample system.
• In immunofluorescence/immunocytochemistry, expect cell membrane and Golgi apparatus staining. Co-localization studies with compartment markers are recommended.
• The immunogen sequence (amino acids 90–189) is conserved across human, mouse, and rat, supporting cross-reactivity in these species; still, verify reactivity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibodies like this NRAS polyclonal antibody, along with bulk ancillary reagents and custom rare-target monoclonal antibody development. Whether advancing oncology diagnostics or research, we bridge your supply needs from concept to clinic.