Anti-NRP2 Polyclonal Antibody for WB, IF/ICC, ELISA - O60462

Core Product Specifications and Parameters

Biological Background: Neuropilin-2 Function and Localization

• Neuropilin-2 (NRP2), also known as VEGF165R2, is a single-pass transmembrane protein that belongs to the neuropilin family. It shares structural features with NRP1, including extracellular CUB and coagulation factor domains, a short transmembrane region, and a cytoplasmic tail.
• NRP2 functions as a high-affinity receptor for class 3 semaphorins (e.g., SEMA3C and SEMA3F) and specific isoforms of vascular endothelial growth factor (VEGF-165 and VEGF-145), as well as the PLGF-2 isoform of placental growth factor. These interactions regulate diverse cellular processes such as axon guidance, angiogenesis, and lymphangiogenesis.
• Beyond its role in normal development, NRP2 acts as a receptor for human cytomegalovirus (HCMV) pentamer-dependent entry in epithelial and endothelial cells, highlighting its involvement in host-virus interactions.
• Subcellularly, NRP2 is localized at the plasma membrane as a transmembrane protein, but it can also be released as a secreted isoform through alternative splicing, enabling non-cell-autonomous functions.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, calcium binding, and heparin binding, all of which modulate its stability, ligand recognition, and signaling.
• Consistent with its multifunctionality, NRP2 is implicated in neurogenesis, developmental differentiation, and pathological processes such as tumor angiogenesis and immune evasion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 21–219) of human NRP2, suggesting the antibody likely targets an epitope within the ligand-binding domain. This may permit detection of both membrane-bound and secreted forms under non-reducing conditions.
• For Western blot, a specific band around 105 kDa is expected. Ensure appropriate sample preparation and consider using a protease inhibitor cocktail to avoid degradation.
• In immunofluorescence (IF/ICC), membrane-localized signal is anticipated. Permeabilization steps may influence staining patterns for intracellular pools; validate with respective controls.
• ELISA performance has been verified; however, optimal coating conditions and detection strategies should be determined empirically for each assay format.
• Cross-reactivity with mouse and rat NRP2 enables translational studies, but species-specific sequence variations may affect affinity. Validate in the relevant sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this NRP2 polyclonal antibody alongside validated antibody pairs, optimized monoclonals, and bulk ancillary reagents tailored to vascular biology and neurodevelopmental research. Our supply network streamlines access to rare-target raw materials, enabling diagnostic manufacturers and labs to accelerate assay development from concept to clinic. For specialized NRP2 applications, CamelBio can source recombinant proteins, matched detection reagents, and technical support to meet your scalable production needs.