Anti-APITD1 Rabbit pAb - Q8N2Z9

Core Product Specifications and Parameters

Biological Background: CENPS/APITD1 Function and Localization

• CENPS (APITD1), also known as MHF1, CENP-S, or FAAP16, is a histone-fold protein that forms heterodimers with CENPX to create the MHF complex, which binds and remodels DNA into nucleosome-like structures. Related references: PMID:20347428, PMID:20347429, PMID:22304917
• As a DNA-binding component of the Fanconi anemia (FA) core complex, CENPS is essential for activation of the FA pathway, promoting monoubiquitination of FANCI-FANCD2 in response to DNA damage and contributing to cellular resistance to cross-linking agents and prevention of chromosomal breakage. Related references: PMID:20347428, PMID:20347429
• In partnership with CENPX and FANCM, CENPS is rapidly recruited to stalled replication forks, where it facilitates gene conversion and replication fork remodeling. Related references: PMID:20347428
• CENPS is a component of the CENP-T-W-S-X heterotetramer, which assembles at centromeres and kinetochores, playing a critical role in kinetochore outer plate formation, microtubule attachment, and faithful chromosome segregation during mitosis. Related references: PMID:19620631
• This protein is ubiquitously expressed in human tissues and localizes to the nucleus, centromere, and kinetochore regions.
• Alternative splicing of CENPS yields multiple isoforms, and the protein is subject to acetylation.
• CENPS does not bind DNA alone; its DNA-binding ability requires heterodimerization with CENPX, with a substrate preference for Holliday junctions, double-stranded DNA, splay arms, and single-stranded DNA in decreasing affinity. Related references: PMID:20347428, PMID:20347429

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against the full-length N-terminal region (aa 1–138) of human CENPS, may detect endogenous protein by WB. Consider using a whole-cell lysate from a human cell line (e.g., HeLa, HEK293) to validate the expected 16 kDa band.
• For ELISA, this antibody can be used as either capture or detection reagent, though specific pairings should be optimized experimentally.
• When probing for CENPS in samples treated with DNA-damaging agents (e.g., mitomycin C), consider including appropriate controls to observe potential changes in expression or localization.
• Always include a loading control and pre-stained molecular weight markers to confirm band size and electrophoretic transfer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supply partner, supporting diagnostic manufacturers and research laboratories with validated antibodies, antigen reagents, and ancillary materials for DNA repair, cell cycle, and kinetochore biology studies. Whether you need this CENPS polyclonal antibody for assay development or require custom monoclonal antibody generation, bulk reagent production, or rare target sourcing, CamelBio streamlines your supply chain from concept to clinic. Contact us to discuss your specific project requirements and take advantage of our integrated CRO and sourcing solutions.