Anti-NPPA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01160

Core Product Specifications and Parameters

Biological Background: NPPA Function and Localization

• NPPA encodes a prohormone that is processed into several bioactive natriuretic peptides, including atrial natriuretic peptide (ANP) and urodilatin, which are key regulators of cardiovascular and renal function.
• The mature ANP hormone binds to the NPR1 receptor and stimulates cGMP production, triggering downstream effectors like PRKG1 to mediate vasodilation, natriuresis, and diuresis. Related references: PMID:1660465, PMID:1672777, PMID:21098034
• It plays a critical role in blood pressure regulation and fluid-electrolyte balance by inhibiting aldosterone synthesis and promoting sodium and water excretion. Related references: PMID:2532366, PMID:2825692, PMID:7595132
• NPPA-derived peptides are also involved in inhibiting cardiac remodeling and hypertrophy by inducing cardiomyocyte apoptosis and suppressing fibroblast growth. Related references: PMID:16875975
• In adipose tissue, ANP regulates lipid metabolism and energy expenditure by activating AMPK and promoting UCP1-dependent thermogenesis in brown fat. Related references: PMID:15741263, PMID:18835931, PMID:21672517
• The protein is secreted and detected in plasma, heart, brain, and kidney; the kidney-specific form urodilatin mediates local sodium and water homeostasis.
• Post-translational modifications include disulfide bond formation and phosphorylation; the precursor (~16 kDa) is cleaved to yield mature peptides.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (aa 1-100) of human NPPA; this region includes the signal peptide and part of the prohormone, and cross-reactivity with mouse and rat suggests epitope conservation.
• For Western blot (WB), expect a band around 16 kDa corresponding to the precursor; shorter exposure may reveal processed forms. Optimize antibody dilution using positive control lysates from heart or kidney tissues.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer, pH 6.0) may be required; validate staining in relevant tissues such as cardiac atria or renal distal tubules.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a suitable matched antibody for sandwich ELISA to quantify NPPA peptides in plasma or urine samples.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio provides a streamlined, one-stop sourcing solution for high-quality antibodies, including this anti-NPPA polyclonal antibody, which supports cardiovascular and renal disease research. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, helping diagnostic manufacturers and researchers move efficiently from concept to clinic.